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Yeast RNA Polymerase II Genes: Isolation with Antibody Probes
Abstract
Genes encoding yeast RNA polymerase II subunits were cloned. Efficient isolation of these genes was accomplished by probing a phage λgt11 recombinant DNA expression library with polyvalent antibodies directed against purified yeast RNA polymerase II. The identity of genes that specify the largest RNA polymerase II subunits, the 220,000- and 150,000-dalton polypeptides, was confirmed by competitive radioimmune assay. Both of these genes exist in single copy in the yeast Saccharomyces cerevisiae .
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Citations
Cited by
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MacBeath, G., & Schreiber, S. L. (2000) · Science · doi:10.1126/science.289.5485.1760 · Cited by 2,767
Sorger, P. K., & Pelham, H. R. B. (1988) · Cell · doi:10.1016/s0092-8674(88)91219-6 · Cited by 832
Query, C. C., Bentley, R. C., & Keene, J. D. (1989) · Cell · doi:10.1016/0092-8674(89)90175-x · Cited by 580
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Beachy, P. A., Helfand, S. L., & Hogness, D. S. (1985) · Nature · doi:10.1038/313545a0 · Cited by 413
Shanklin, J., & Somerville, C. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.6.2510 · Cited by 334
Metadata
- Authors
- Young, R. A., & Davis, R. W.
- Venue
- Science
- Published
- 1983-11-18
- Volume
- 222
- Issue
- 4625
- Pages
- 778-782
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2080795403
- Cited by
- 1,061
- References
- 0
- FWCI
- 34.65
- Type
- journal article
- Language
- en
- Open access
- closed
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- Young, R. A., & Davis, R. W. (1983). Yeast RNA Polymerase II Genes: Isolation with Antibody Probes. Science. https://doi.org/10.1126/science.6356359
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
