The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Biological Activity of Recombinant Human Interleukin-2 Produced in Escherichia coli
Abstract
The gene for interleukin-2 was isolated from the Jurkat cell line and from normal peripheral blood lymphocytes and, when inserted in Escherichia coli , was expressed at high concentrations. This interleukin-2 was purified to apparent homogeneity and tested for biological activity in a variety of assays in vitro and in vivo. The recombinant lymphokine supports the growth of murine and human interleukin-2 dependent cell lines, enhances the generation of murine and human cytolytic cells in vitro, and generates lymphokine activated killer cells from murine and human lymphocytes. It has a serum half-life of 2 to 3 minutes in the mouse and significantly enhances the generation of cytolytic cells in vivo after alloimmunization. No functional differences between native and the recombinant interleukin-2 molecules have been detected.
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Citations
Cites
Berger, S. L., & Birkenmeier, C. S. (1979) · Biochemistry · doi:10.1021/bi00590a018 · Cited by 443
Cited by
Rosenberg, S. A., Packard, B. S., Aebersold, P. M., Solomon, D., Topalian, S. L., Toy, S. T., Simon, P., Lotze, M. T., Yang, J. C., Seipp, C. A., et al. (1988) · New England Journal of Medicine · doi:10.1056/nejm198812223192527 · Cited by 2,389
Self-citationMulé, J. J., Shu, S., Schwarz, S. L., & Rosenberg, S. A. (1984) · Science · doi:10.1126/science.6332379 · Cited by 688
Self-citationCAR T cells: Continuation in a revolution of immunotherapy
Singh, A. K., & McGuirk, J. P. (2020) · The Lancet Oncology · doi:10.1016/s1470-2045(19)30823-x · Cited by 303
Interleukin-2: Clinical applications
Atkins, M. B. (2002) · Seminars in Oncology · doi:10.1053/sonc.2002.33077 · Cited by 156
Large scale production of human lymphokine activated killer cells for use in adoptive immunotherapy
Mesler Muul, L., Director, E. P., Hyatt, C. L., & Rosenberg, S. A. (1986) · Journal of Immunological Methods · doi:10.1016/0022-1759(86)90015-3 · Cited by 82
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Metadata
- Authors
- Rosenberg, S. A., Grimm, E. A., McGrogan, M., Doyle, M., Kawasaki, E., Koths, K., & Mark, D. F.
- Venue
- Science
- Published
- 1984-03-30
- Volume
- 223
- Issue
- 4643
- Pages
- 1412-1415
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2012913493
- Cited by
- 595
- References
- 1
- FWCI
- 32.29
- Type
- journal article
- Language
- en
- Open access
- closed
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- Rosenberg, S. A., Grimm, E. A., McGrogan, M., Doyle, M., Kawasaki, E., Koths, K., & Mark, D. F. (1984). Biological Activity of Recombinant Human Interleukin-2 Produced in Escherichia coli. Science. https://doi.org/10.1126/science.6367046
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
