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Cited by 347

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Cloning Human Fetal γ Globin and Mouse α-Type Globin DNA: Preparation and Screening of Shotgun Collections

Blattner, Frederick R., Blechl, Ann E., Denniston-Thompson, Katherine, Faber, Harvey E., Richards, Julia E., Slightom, Jerry L., Tucker, Phillip W., Smithies, Oliver·1978·Science

Abstract

Shotgun collections of Charon 3A bacteriophages containing Eco RI fragments of human and mouse DNA were constructed with the use of in vitro packaging. Plaques were screened by hybridization, and globin-specific clones were isolated from both human (Charon 3AHs51.1) and mouse (Charon 3AMm30.5). The fragments cloned were detected in unfractionated genomic DNA by the Southern method of hybridization.

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Metadata

Authors
Blattner, F. R., Blechl, A. E., Denniston-Thompson, K., Faber, H. E., Richards, J. E., Slightom, J. L., Tucker, P. W., & Smithies, O.
Venue
Science
Published
1978-12-22
Volume
202
Issue
4374
Pages
1279-1284
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2007672823
Cited by
347
References
0
FWCI
35.64
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Cloning Human Fetal γ Globin and Mouse α-Type Globin DNA: Preparation and Screening of Shotgun Collections. https://app.cochat.ai/papers/10.1126/science.725603
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Blattner, F. R., Blechl, A. E., Denniston-Thompson, K., Faber, H. E., Richards, J. E., Slightom, J. L., Tucker, P. W., & Smithies, O. (1978). Cloning Human Fetal γ Globin and Mouse α-Type Globin DNA: Preparation and Screening of Shotgun Collections. Science. https://doi.org/10.1126/science.725603

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.