The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Alignment and Sensitive Detection of DNA by a Moving Interface
Abstract
In a process called "molecular combing," DNA molecules attached at one end to a solid surface were extended and aligned by a receding air-water interface and left to dry on the surface. Molecular combing was observed to extend the length of the bacteriophage λ DNA molecule to 21.5 ± 0.5 micrometers (unextended length, 16.2 micrometers). With the combing process, it was possible to (i) extend a chromosomal Escherichia coli DNA fragment (10 6 base pairs) and (ii) detect a minute quantity of DNA (10 3 molecules). These results open the way for a faster physical mapping of the genome and for the detection of small quantities of target DNA from a population of molecules.
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Cites
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
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Kasumov, A. Y., Kociak, M., Guéron, S., Reulet, B., Volkov, V. T., Klinov, D. V., & Bouchiat, H. (2001) · Science · doi:10.1126/science.291.5502.280 · Cited by 710
Preventing replication fork collapse to maintain genome integrity
Cortez, D. (2015) · DNA Repair · doi:10.1016/j.dnarep.2015.04.026 · Cited by 223
Fibrillin Microfibrils are Stiff Reinforcing Fibres in Compliant Tissues
Sherratt, M. J., Baldock, C., Louise Haston, J., Holmes, D. F., Jones, C. J. P., Adrian Shuttleworth, C., Wess, T. J., & Kielty, C. M. (2003) · Journal of Molecular Biology · doi:10.1016/s0022-2836(03)00829-5 · Cited by 147
Human ribosomal RNA gene arrays display a broad range of palindromic structures
Caburet, S., Conti, C., Schurra, C., Lebofsky, R., Edelstein, S. J., & Bensimon, A. (2005) · Genome Research · doi:10.1101/gr.3970105 · Cited by 143
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Metadata
- Authors
- Bensimon, A., Simon, A., Chiffaudel, A., Croquette, V., Heslot, F., & Bensimon, D.
- Venue
- Science
- Published
- 1994-09-30
- Volume
- 265
- Issue
- 5181
- Pages
- 2096-2098
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1971701163
- Cited by
- 843
- References
- 1
- FWCI
- 4.53
- Type
- journal article
- Language
- en
- Open access
- closed
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- Bensimon, A., Simon, A., Chiffaudel, A., Croquette, V., Heslot, F., & Bensimon, D. (1994). Alignment and Sensitive Detection of DNA by a Moving Interface. Science. https://doi.org/10.1126/science.7522347
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
