The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Antisense Gene Inhibition by Oligonucleotides Containing C-5 Propyne Pyrimidines
Abstract
Phosphorothioate oligodeoxynucleotides containing the C-5 propyne analogs of uridine and cytidine bind RNA with high affinity and are potent antisense inhibitors of gene expression. In a cellular assay, gene-specific antisense inhibition occurred at nanomolar concentrations of oligonucleotide, was dose-dependent and exquisitely sensitive to sequence mismatches, and was correlated with the melting temperature and length of oligonucleotide. Activity was independent of RNA target site and cell type but was detectable only when the oligonucleotides were microinjected or delivered with cell-permeabilizing agents. These oligonucleotides may have important applications in therapy and in studies of gene function.
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Cites
Antisense and Antigene Properties of Peptide Nucleic Acids
Hanvey, J. C., Peffer, N. J., Bisi, J. E., Thomson, S. A., Cadilla, R., Josey, J. A., Ricca, D. J., Hassman, C. F., Bonham, M. A., Au, K. G., et al. (1992) · Science · doi:10.1126/science.1279811 · Cited by 533
Chiang, M. Y., Chan, H., Zounes, M. A., Freier, S. M., Lima, W. F., & Bennett, C. F. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55250-9 · Cited by 463
Anfossi, G., Gewirtz, A. M., & Calabretta, B. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.9.3379 · Cited by 270
Fisher, T. L., Terhorst, T., Cao, X., & Wagner, R. W. (1993) · Nucleic Acids Research · doi:10.1093/nar/21.16.3857 · Cited by 240
Self-citationOligodeoxynucleotides containing C-5 propyne analogs of 2′-deoxyuridine and 2′-deoxycytidine
Froehler, B. C., Wadwani, S., Terhorst, T. J., & Gerrard, S. R. (1992) · Tetrahedron Letters · doi:10.1016/s0040-4039(00)79079-4 · Cited by 183
Self-citationSimons, M., & Rosenberg, R. D. (1992) · Circulation Research · doi:10.1161/01.res.70.4.835 · Cited by 171
[30] Microinjection of tissue culture cells
Graessmann, M., & Graessmann, A. (1983) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(83)01033-2 · Cited by 149
Protocols for Oligonucleotides and Analogs
Agrawal, S. (1993) · Humana Press eBooks · doi:10.1385/0896032817 · Cited by 127
Phosphorothioate oligodeoxynucleotides—anti-sense inhibitors of gene expression?
Stein, C. A., Tonkinson, J. L., & Yakubov, L. A. (1991) · Pharmacology & Therapeutics · doi:10.1016/0163-7258(91)90032-h · Cited by 108
Rosolen, A., Whitesell, L. J., Ikegaki, N., Kennett, R. H., & Neckers, L. M. (1990) · PubMed · Cited by 55
Cited by
Bennett, C. F., & Swayze, E. E. (2010) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pharmtox.010909.105654 · Cited by 1,374
Morpholino Antisense Oligomers: Design, Preparation, and Properties
SUMMERTON, J., & WELLER, D. (1997) · Antisense and Nucleic Acid Drug Development · doi:10.1089/oli.1.1997.7.187 · Cited by 1,116
Bielinska, A. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.11.2176 · Cited by 451
The Medicinal Chemistry of Therapeutic Oligonucleotides
Wan, W. B., & Seth, P. P. (2016) · Journal of Medicinal Chemistry · doi:10.1021/acs.jmedchem.6b00551 · Cited by 429
Progress in antisense technology
Crooke, S. T. (2004) · Annual Review of Medicine · doi:10.1146/annurev.med.55.091902.104408 · Cited by 417
Metadata
- Authors
- Wagner, R. W., Matteucci, M. D., Lewis, J. G., Gutierrez, A. J., Moulds, C., & Froehler, B. C.
- Venue
- Science
- Published
- 1993-06-04
- Volume
- 260
- Issue
- 5113
- Pages
- 1510-1513
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1981481692
- Cited by
- 405
- References
- 13
- FWCI
- 11.99
- Type
- journal article
- Language
- en
- Open access
- closed
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- Wagner, R. W., Matteucci, M. D., Lewis, J. G., Gutierrez, A. J., Moulds, C., & Froehler, B. C. (1993). Antisense Gene Inhibition by Oligonucleotides Containing C-5 Propyne Pyrimidines. Science. https://doi.org/10.1126/science.7684856
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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