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Gene Trap Tagging of PROLIFERA , an Essential MCM2-3-5 -Like Gene in Arabidopsis

Springer, Patricia S., McCombie, W. Richard, Sundaresan, Venkatesan, Martienssen, Robert A.·1995·Science

Abstract

Gene trap transposon mutagenesis can identify essential genes whose functions in later development are obscured by an early lethal phenotype. In higher plants, many genes are required for haploid gametophyte viability, so that the phenotypic effects of their disruption cannot be readily observed in the diploid plant body. The PROLIFERA ( PRL ) gene, identified by gene trap transposon mutagenesis in Arabidopsis , is required for megagametophyte and embryo development. Reporter gene expression patterns revealed that PRL was expressed in dividing cells throughout the plant. PRL is related to the MCM2-3-5 family of yeast genes that are required for the initiation of DNA replication.

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Metadata

Authors
Springer, P. S., McCombie, W. R., Sundaresan, V., & Martienssen, R. A.
Venue
Science
Published
1995-05-12
Volume
268
Issue
5212
Pages
877-880
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2046177801
Cited by
257
References
0
FWCI
30.79
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Gene Trap Tagging of PROLIFERA , an Essential MCM2-3-5 -Like Gene in Arabidopsis. https://app.cochat.ai/papers/10.1126/science.7754372
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Springer, P. S., McCombie, W. R., Sundaresan, V., & Martienssen, R. A. (1995). Gene Trap Tagging of PROLIFERA , an Essential MCM2-3-5 -Like Gene in Arabidopsis. Science. https://doi.org/10.1126/science.7754372

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