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Gene Trap Tagging of PROLIFERA , an Essential MCM2-3-5 -Like Gene in Arabidopsis
Abstract
Gene trap transposon mutagenesis can identify essential genes whose functions in later development are obscured by an early lethal phenotype. In higher plants, many genes are required for haploid gametophyte viability, so that the phenotypic effects of their disruption cannot be readily observed in the diploid plant body. The PROLIFERA ( PRL ) gene, identified by gene trap transposon mutagenesis in Arabidopsis , is required for megagametophyte and embryo development. Reporter gene expression patterns revealed that PRL was expressed in dividing cells throughout the plant. PRL is related to the MCM2-3-5 family of yeast genes that are required for the initiation of DNA replication.
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Cited by
Agrobacterium -Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool
Gelvin, S. B. (2003) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.67.1.16-37.2003 · Cited by 1,379
The Lateral Organ Boundaries Gene Defines a Novel, Plant-Specific Gene Family
Shuai, B., Reynaga-Peña, C. G., & Springer, P. S. (2002) · Plant Physiology · doi:10.1104/pp.010926 · Cited by 555
Self-citationFemale Gametophyte Development
Yadegari, R. (2004) · THE PLANT CELL ONLINE · doi:10.1105/tpc.018192 · Cited by 451
ASYMMETRIC LEAVES1 reveals knox gene redundancy in Arabidopsis
Byrne, M. E., Simorowski, J., & Martienssen, R. A. (2002) · Development · doi:10.1242/dev.129.8.1957 · Cited by 406
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Metadata
- Authors
- Springer, P. S., McCombie, W. R., Sundaresan, V., & Martienssen, R. A.
- Venue
- Science
- Published
- 1995-05-12
- Volume
- 268
- Issue
- 5212
- Pages
- 877-880
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2046177801
- Cited by
- 257
- References
- 0
- FWCI
- 30.79
- Type
- journal article
- Language
- en
- Open access
- closed
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- Springer, P. S., McCombie, W. R., Sundaresan, V., & Martienssen, R. A. (1995). Gene Trap Tagging of PROLIFERA , an Essential MCM2-3-5 -Like Gene in Arabidopsis. Science. https://doi.org/10.1126/science.7754372
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