The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Inducible Gene Expression in Trypanosomes Mediated by a Prokaryotic Repressor
Abstract
An inducible expression system was developed for the protozoan parasite Trypanosoma brucei . Transgenic trypanosomes expressing the tetracycline repressor of Escherichia coli exhibited inducer (tetracycline)-dependent expression of chromosomally integrated reporter genes under the control of a procyclic acidic repetitive protein (PARP) promoter bearing a tet operator. Reporter expression could be controlled over a range of four orders of magnitude in response to tetracycline concentration, a degree of regulation that exceeds those exhibited by other eukaryotic repression-based systems. The tet repressor-controlled PARP promoter should be a valuable tool for the study of trypanosome biochemistry, pathogenicity, and cell and molecular biology.
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Citations
Cites
Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
Gossen, M., & Bujard, H. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.12.5547 · Cited by 4,943
Evolutionary diversity of eukaryotic small-subunit rRNA genes.
Sogin, M. L., Elwood, H. J., & Gunderson, J. H. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.5.1383 · Cited by 492
Trans splicing of nuclear pre-mRNAs
Agabian, N. (1990) · Cell · doi:10.1016/0092-8674(90)90674-4 · Cited by 386
Tubulin genes are tandemly linked and clustered in the genome of trypanosoma brucei
Thomashow, L. S., Milhausen, M., Rutter, W. J., & Agabian, N. (1983) · Cell · doi:10.1016/0092-8674(83)90494-4 · Cited by 253
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Kooter, J. M., & Borst, P. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.24.9457 · Cited by 216
The inducible Iac operator-repressor system is functional in mammalian cells
HU, M. (1987) · Cell · doi:10.1016/0092-8674(87)90234-0 · Cited by 207
The promoter for a variant surface glycoprotein gene expression site in Trypanosoma brucei.
Zomerdijk, J. C., Ouellette, M., ten Asbroek, A. L., Kieft, R., Bommer, A. M., Clayton, C. E., & Borst, P. (1990) · The EMBO Journal · doi:10.1002/j.1460-2075.1990.tb07467.x · Cited by 205
Self-citationRoditi, I., Carrington, M., & Turner, M. (1987) · Nature · doi:10.1038/325272a0 · Cited by 201
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Cited by
Wang, Z., Morris, J. C., Drew, M. E., & Englund, P. T. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m008405200 · Cited by 517
RNA interference in protozoan parasites: Protozoan parasites and RNAi
Ullu, E., Tschudi, C., & Chakraborty, T. (2004) · Cellular Microbiology · doi:10.1111/j.1462-5822.2004.00399.x · Cited by 290
A toolkit enabling efficient, scalable and reproducible gene tagging in trypanosomatids
Dean, S., Sunter, J., Wheeler, R. J., Hodkinson, I., Gluenz, E., & Gull, K. (2015) · Open Biology · doi:10.1098/rsob.140197 · Cited by 264
Berens, C., & Hillen, W. (2003) · European Journal of Biochemistry · doi:10.1046/j.1432-1033.2003.03694.x · Cited by 261
Metadata
- Authors
- Wirtz, E., & Clayton, C.
- Venue
- Science
- Published
- 1995-05-26
- Volume
- 268
- Issue
- 5214
- Pages
- 1179-1183
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2036780183
- Cited by
- 255
- References
- 25
- FWCI
- 11.59
- Type
- journal article
- Language
- en
- Open access
- closed
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- Wirtz, E., & Clayton, C. (1995). Inducible Gene Expression in Trypanosomes Mediated by a Prokaryotic Repressor. Science. https://doi.org/10.1126/science.7761835
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
