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Crystal Structure of a Purple Acid Phosphatase Containing a Dinuclear Fe(III)-Zn(II) Active Site
Abstract
Kidney bean purple acid phosphatase (KBPAP) is an Fe(III)-Zn(II) metalloenzyme resembling the mammalian Fe(III)-Fe(II) purple acid phosphatases. The structure of the homodimeric 111-kilodalton KBPAP was determined at a resolution of 2.9 angstroms. The enzyme contains two domains in each subunit. The active site is located in the carboxyl-terminal domain at the carboxy end of two sandwiched βαβαβ motifs. The two metal ions are 3.1 angstroms apart and bridged monodentately by Asp 164 . The iron is further coordinated by Tyr 167 , His 325 , and Asp 135 , and the zinc by His 286 , His 323 , and Asn 201 . The active-site structure is consistent with previous proposals regarding the mechanism of phosphate ester hydrolysis involving nucleophilic attack on the phosphate group by an Fe(III)-coordinated hydroxide ion.
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Citations
Cited by
Jasniewski, A. J., & Que, L. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00457 · Cited by 474
Trace metal metabolism in plants
Andresen, E., Peiter, E., & Küpper, H. (2018) · Journal of Experimental Botany · doi:10.1093/jxb/erx465 · Cited by 431
Purple Acid Phosphatases of Arabidopsis thaliana
Li, D., Zhu, H., Liu, K., Liu, X., Leggewie, G., Udvardi, M., & Wang, D. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m204183200 · Cited by 287
Metadata
- Authors
- Sträter, N., Klabunde, T., Tucker, P., Witzel, H., & Krebs, B.
- Venue
- Science
- Published
- 1995-06-09
- Volume
- 268
- Issue
- 5216
- Pages
- 1489-1492
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2020386376
- Cited by
- 434
- References
- 0
- FWCI
- 34.74
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Crystal Structure of a Purple Acid Phosphatase Containing a Dinuclear Fe(III)-Zn(II) Active Site. https://app.cochat.ai/papers/10.1126/science.7770774
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- Sträter, N., Klabunde, T., Tucker, P., Witzel, H., & Krebs, B. (1995). Crystal Structure of a Purple Acid Phosphatase Containing a Dinuclear Fe(III)-Zn(II) Active Site. Science. https://doi.org/10.1126/science.7770774
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