The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Transcriptional Activation by Tetracyclines in Mammalian Cells
Abstract
A transcriptional transactivator was developed that fuses the VP16 activation domain with a mutant Tet repressor from Escherichia coli. This transactivator requires certain tetracycline (Tc) derivatives for specific DNA binding. Thus, addition of doxycycline to HeLa cells that constitutively synthesized the transactivator and that contained an appropriate, stably integrated reporter unit rapidly induced gene expression more than a thousandfold. The specificity of the Tet repressor-operator-effector interaction and the pharmacological characteristics of Tc's make this regulatory system well suited for the control of gene activities in vivo, such as in transgenic animals and possibly in gene therapy.
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In the citing paper
“In 1995, Gossen et al. developed the Tet-On system (Fig. 1C), a regulatory system that allows activation of gene expression by the addition - instead of removal - of dox [12].”
From Tet-On Systems For Doxycycline-inducible Gene Expression (2016) · cites this paper as [12]
In the citing paper
“This reverse-TetR (rTetR) differed at four amino acid positions from the original protein (Fig. 2).”
From Tet-On Systems For Doxycycline-inducible Gene Expression (2016) · cites this paper as [12]
Citations
Cites
Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
Gossen, M., & Bujard, H. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.12.5547 · Cited by 4,943
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Research works printed but not linked: 13
- 10.1016/0003-2697(87)90021-2 · DOI
- 10.1016/0003-2697(83)90418-9 · DOI
- FURTH, P.A., TEMPORAL CONTROL OF GENE-EXPRESSION IN TRANSGENIC MICE BY A TETRACYCLINE-RESPONSIVE PROMOTER, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 91: 9302 (1994).
- GOSSEN, M, CONTROL OF GENE ACTIVITY IN HIGHER EUKARYOTIC CELLS BY PROKARYOTIC REGULATORY ELEMENTS, TRENDS IN BIOCHEMICAL SCIENCES 18: 471 (1993). · DOI
- HECHT, B, NONINDUCIBLE TET REPRESSOR MUTATIONS MAP FROM THE OPERATOR BINDING MOTIF TO THE C-TERMINUS, JOURNAL OF BACTERIOLOGY 175: 1206 (1993). · DOI
- HILLEN, W, MECHANISMS UNDERLYING EXPRESSION OF TN10 ENCODED TETRACYCLINE RESISTANCE, ANNUAL REVIEW OF MICROBIOLOGY 48: 345 (1994). · DOI · Paper page
- HINRICHS, W, STRUCTURE OF THE TET REPRESSOR TETRACYCLINE COMPLEX AND REGULATION OF ANTIBIOTIC-RESISTANCE, SCIENCE 264: 418 (1994). · DOI · Paper page
- MULLER G THESIS U ERLANGEN NU (1995).
- RESNITZKY, D, ACCELERATION OF THE G(1)/S PHASE-TRANSITION BY EXPRESSION OF CYCLIN-D1 AND CYCLIN-E WITH AN INDUCIBLE SYSTEM, MOLECULAR AND CELLULAR BIOLOGY 14: 1669 (1994).
- 10.1073/pnas.81.7.1991 · DOI
- SOUTHERN, P.J., J MOL APPL GENET 1: 327 (1982).
- WEINMANN, P, A CHIMERIC TRANSACTIVATOR ALLOWS TETRACYCLINE-RESPONSIVE GENE-EXPRESSION IN WHOLE PLANTS, PLANT JOURNAL 5: 559 (1994). · DOI
- YARRANTON, G.T., CURR OPIN BIOTECH 3: 506 (1992). · DOI
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Comijn, J., Berx, G., Vermassen, P., Verschueren, K., van Grunsven, L. V., Bruyneel, E., Mareel, M., Huylebroeck, D., & van Roy, F. (2001) · Molecules and Cells · doi:10.1016/s1097-2765(01)00260-x · Cited by 1,464
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Metadata
- Authors
- Gossen, M., Freundlieb, S., Bender, G., Müller, G., Hillen, W., & Bujard, H.
- Affiliations
- Heidelberg University (DE)
- ORCID
- Gossen, Manfred
- Venue
- Science
- Published
- 1995-06-23
- Volume
- 268
- Issue
- 5218
- Pages
- 1766-1769
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2005275397
- Cited by
- 2,488
- References
- 0
- Citations by year
- 2026: 31 · 2025: 43 · 2024: 45 · 2023: 49 · 2022: 55 · 2021: 60 · 2020: 68 · 2019: 52 · 2018: 55 · 2017: 47 · 2016: 52 · 2015: 62 · 2014: 53 · 2013: 67 · 2012: 64
- FWCI
- 48.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Keywords
- tetracycline, transcriptional activation, Tet repressor, VP16 activation domain, doxycycline, gene expression regulation, transcriptional activator, transgenic animals, gene therapy
- MeSH terms
- Amino Acid Sequence, Doxycycline, HeLa Cells, Humans, Molecular Sequence Data, Operator Regions, Genetic, Recombinant Fusion Proteins, Repressor Proteins, Transfection, Viral Proteins, Transcriptional Activation, Trans-Activators, Genes, Reporter, Simplexvirus
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