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Synthesis of Proteins by Native Chemical Ligation
Abstract
A simple technique has been devised that allows the direct synthesis of native backbone proteins of moderate size. Chemoselective reaction of two unprotected peptide segments gives an initial thioester-linked species. Spontaneous rearrangement of this transient intermediate yields a full-length product with a native peptide bond at the ligation site. The utility of native chemical ligation was demonstrated by the one-step preparation of a cytokine containing multiple disulfides. The polypeptide ligation product was folded and oxidized to form the native disulfide-containing protein molecule. Native chemical ligation is an important step toward the general application of chemistry to proteins.
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Cited by
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Bazan, J. F., Bacon, K. B., Hardiman, G., Wang, W., Soo, K., Rossi, D., Greaves, D. R., Zlotnik, A., & Schall, T. J. (1997) · Nature · doi:10.1038/385640a0 · Cited by 2,013
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Kowada, T., Maeda, H., & Kikuchi, K. (2015) · Chemical Society Reviews · doi:10.1039/c5cs00030k · Cited by 1,381
Harrison, J. K., Jiang, Y., Chen, S., Xia, Y., Maciejewski, D., McNamara, R. K., Streit, W. J., Salafranca, M. N., Adhikari, S., Thompson, D. A., et al. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.18.10896 · Cited by 1,176
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Metadata
- Authors
- Dawson, P. E., Muir, T. W., Clark-Lewis, I., & Kent, S. B. H.
- Venue
- Science
- Published
- 1994-11-04
- Volume
- 266
- Issue
- 5186
- Pages
- 776-779
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W4213262935
- Cited by
- 4,131
- References
- 0
- FWCI
- 9.68
- Type
- journal article
- Language
- en
- Open access
- closed
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- Dawson, P. E., Muir, T. W., Clark-Lewis, I., & Kent, S. B. H. (1994). Synthesis of Proteins by Native Chemical Ligation. Science. https://doi.org/10.1126/science.7973629
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
