The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Heat-Inducible Degron: a Method for Constructing Temperature-Sensitive Mutants
Abstract
A temperature-sensitive ( ts ) mutant retains the function of a gene at a low (permissive) temperature but not at a high (nonpermissive) temperature. Arg-DHFR, a dihydrofolate reductase bearing an amino-terminal (N-terminal) arginine, is long-lived in the yeast Saccharomyces cerevisiae , even though arginine is a destabilizing residue in the N-end rule of protein degradation. A ts derivative of Arg-DHFR was identified that is long-lived at 23°C but rapidly degraded by the N-end rule pathway at 37°C. Fusions of ts Arg-DHFR to either Ura3 or Cdc28 of S. cerevisiae confer ts phenotypes specific for these gene products. Thus, Arg-DHFR ts is a heat-inducible degradation signal that can be used to produce ts mutants without a search for ts mutations.
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Sensing DNA Damage Through ATRIP Recognition of RPA-ssDNA Complexes
Zou, L., & Elledge, S. J. (2003) · Science · doi:10.1126/science.1083430 · Cited by 2,693
An auxin-based degron system for the rapid depletion of proteins in nonplant cells
Nishimura, K., Fukagawa, T., Takisawa, H., Kakimoto, T., & Kanemaki, M. (2009) · Nature Methods · doi:10.1038/nmeth.1401 · Cited by 1,663
Banaszynski, L. A., Chen, L.-C., Maynard-Smith, L. A., Ooi, A. G. L., & Wandless, T. J. (2006) · Cell · doi:10.1016/j.cell.2006.07.025 · Cited by 902
The N-end rule: functions, mysteries, uses
Varshavsky, A. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.22.12142 · Cited by 859
Self-citationRapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors
Natsume, T., Kiyomitsu, T., Saga, Y., & Kanemaki, M. T. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.03.001 · Cited by 673
Metadata
- Authors
- Dohmen, R. J., Wu, P., & Varshavsky, A.
- Venue
- Science
- Published
- 1994-03-04
- Volume
- 263
- Issue
- 5151
- Pages
- 1273-1276
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2048599311
- Cited by
- 347
- References
- 33
- FWCI
- 3.31
- Type
- journal article
- Language
- en
- Open access
- closed
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- Dohmen, R. J., Wu, P., & Varshavsky, A. (1994). Heat-Inducible Degron: a Method for Constructing Temperature-Sensitive Mutants. Science. https://doi.org/10.1126/science.8122109
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
