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Cited by 366

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Release of Active Cytokinin by a β-Glucosidase Localized to the Maize Root Meristem

Brzobohatý, Břetislav, Moore, Ian, Kristoffersen, Peter, Bako, Laszlo, Campos, Narciso, Schell, Jeff, Palme, Klaus·1993·Science

Abstract

A β-glucoside encoded by a cloned Zea mays complementary DNA ( Zm-p60.1 ) cleaved the biologically inactive hormone conjugates cytokinin-O-glucosides and kinetin-N3-glucoside, releasing active cytokinin. Tobacco protoplasts that transiently expressed Zm-p60.1 could use the inactive cytokinin glucosides to initiate cell division. The ability of protoplasts to sustain growth in response to cytokinin glucosides persisted indefinitely after the likely disappearance of the expression vector. In the roots of maize seedlings, Zm-p60.1 was localized to the meristematic cells and may function in vivo to supply the developing maize embryo with active cytokinin.

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Authors
Brzobohatý, B., Moore, I., Kristoffersen, P., Bako, L., Campos, N., Schell, J., & Palme, K.
Venue
Science
Published
1993-11-12
Volume
262
Issue
5136
Pages
1051-1054
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2020644185
Cited by
366
References
0
FWCI
18.38
Type
journal article
Language
en
Open access
closed

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