The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Signal Sequence Trap: a Cloning Strategy for Secreted Proteins and Type I Membrane Proteins
Abstract
A method was developed to clone, without the use of specific functional assays, complementary DNAs (cDNAs) that carry specific amino-terminal signal sequences, such as those encoding intercellular signal-transducing molecules and receptors. The vector used in this system directed the cell surface expression of interleukin-2 receptor fusion proteins when inserts with signal sequences were cloned in-frame with the correct orientation. An expression cDNA library was constructed from a bone marrow stromal cell line, which contained 5′ portion-enriched cDNAs (the average size was 400 base pairs). Two cDNAs that encoded putative cytokine molecules, stromal cell-derived factor-1α (SDF-1α) and SDF-1β, which belong to the intercrine-macrophage inflammatory protein superfamily, were cloned.
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Identification of a receptor for the morphogen retinoic acid
Giguere, V., Ong, E. S., Segui, P., & Evans, R. M. (1987) · Nature · doi:10.1038/330624a0 · Cited by 1,965
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Murphy, P. M., Baggiolini, M., Charo, I. F., Hébert, C. A., Horuk, R., Matsushima, K., Miller, L. H., Oppenheim, J. J., & Power, C. A. (2000) · Pharmacological Reviews · doi:10.1016/s0031-6997(24)01439-x · Cited by 1,817
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Bleul, C. C., Fuhlbrigge, R. C., Casasnovas, J. M., Aiuti, A., & Springer, T. A. (1996) · The Journal of experimental medicine · doi:10.1084/jem.184.3.1101 · Cited by 1,482
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Metadata
- Authors
- Tashiro, K., Tada, H., Heilker, R., Shirozu, M., Nakano, T., & Honjo, T.
- Venue
- Science
- Published
- 1993-07-30
- Volume
- 261
- Issue
- 5121
- Pages
- 600-603
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2060686995
- Cited by
- 713
- References
- 2
- FWCI
- 4.01
- Type
- journal article
- Language
- en
- Open access
- closed
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- Tashiro, K., Tada, H., Heilker, R., Shirozu, M., Nakano, T., & Honjo, T. (1993). Signal Sequence Trap: a Cloning Strategy for Secreted Proteins and Type I Membrane Proteins. Science. https://doi.org/10.1126/science.8342023
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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