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Cited by 713

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Signal Sequence Trap: a Cloning Strategy for Secreted Proteins and Type I Membrane Proteins

Tashiro, Kei, Tada, Hideaki, Heilker, Ralf, Shirozu, Michio, Nakano, Toru, Honjo, Tasuku·1993·Science

Abstract

A method was developed to clone, without the use of specific functional assays, complementary DNAs (cDNAs) that carry specific amino-terminal signal sequences, such as those encoding intercellular signal-transducing molecules and receptors. The vector used in this system directed the cell surface expression of interleukin-2 receptor fusion proteins when inserts with signal sequences were cloned in-frame with the correct orientation. An expression cDNA library was constructed from a bone marrow stromal cell line, which contained 5′ portion-enriched cDNAs (the average size was 400 base pairs). Two cDNAs that encoded putative cytokine molecules, stromal cell-derived factor-1α (SDF-1α) and SDF-1β, which belong to the intercrine-macrophage inflammatory protein superfamily, were cloned.

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Crossref · 10.1126/science.8342023

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Metadata

Authors
Tashiro, K., Tada, H., Heilker, R., Shirozu, M., Nakano, T., & Honjo, T.
Venue
Science
Published
1993-07-30
Volume
261
Issue
5121
Pages
600-603
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2060686995
Cited by
713
References
2
FWCI
4.01
Type
journal article
Language
en
Open access
closed

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

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