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Activation of the Sphingomyelin Signaling Pathway in Intact EL4 Cells and in a Cell-Free System By IL-1β
Abstract
The mechanism of interleukin-1 (IL-1) signaling is unknown. Tumor necrosis factor-α uses a signal transduction pathway that involves sphingomyelin hydrolysis to ceramide and stimulation of a ceramide-activated protein kinase. In intact EL4 thymoma cells, IL-1β similarly stimulated a rapid decrease of sphingomyelin and an elevation of ceramide, and enhanced ceramide-activated protein kinase activity. This cascade was also activated by IL-1β in a cell-free system, demonstrating tight coupling to the receptor. Exogenous sphingomyelinase, but not phospholipases A 2 , C, or D, in combination with phorbol ester replaced IL-1β to stimulate IL-2 secretion. Thus, IL-1β signals through the sphingomyelin pathway.
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Cited by
Activation of the nuclear factor-kappaB by Rho, CDC42, and Rac-1 proteins
Perona, R., Montaner, S., Saniger, L., Sánchez-Pérez, I., Bravo, R., & Lacal, J. C. (1997) · Genes & Development · doi:10.1101/gad.11.4.463 · Cited by 519
Ericsson, A., Liu, C., Hart, R. P., & Sawchenko, P. E. (1995) · Journal of Comparative Neurology · doi:10.1002/cne.903610410 · Cited by 471
How do protein kinases recognize their substrates?
Pinna, L. A., & Ruzzene, M. (1996) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/s0167-4889(96)00083-3 · Cited by 470
Acid sphingomyelinase–ceramide system mediates effects of antidepressant drugs
Gulbins, E., Palmada, M., Reichel, M., Lüth, A., Böhmer, C., Amato, D., Müller, C. P., Tischbirek, C. H., Groemer, T. W., Tabatabai, G., et al. (2013) · Nature Medicine · doi:10.1038/nm.3214 · Cited by 384
Acidic Sphingomyelinase Mediates Entry of N. gonorrhoeae into Nonphagocytic Cells
Grassmé, H., Gulbins, E., Brenner, B., Ferlinz, K., Sandhoff, K., Harzer, K., Lang, F., & Meyer, T. F. (1997) · Cell · doi:10.1016/s0092-8674(00)80448-1 · Cited by 320
Metadata
- Authors
- Mathias, S., Younes, A., Kan, C.-C., Orlow, I., Joseph, C., & Kolesnick, R. N.
- Venue
- Science
- Published
- 1993-01-22
- Volume
- 259
- Issue
- 5094
- Pages
- 519-522
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2080071635
- Cited by
- 433
- References
- 0
- FWCI
- 21.23
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Activation of the Sphingomyelin Signaling Pathway in Intact EL4 Cells and in a Cell-Free System By IL-1β. https://app.cochat.ai/papers/10.1126/science.8424175
- The paper
- Mathias, S., Younes, A., Kan, C.-C., Orlow, I., Joseph, C., & Kolesnick, R. N. (1993). Activation of the Sphingomyelin Signaling Pathway in Intact EL4 Cells and in a Cell-Free System By IL-1β. Science. https://doi.org/10.1126/science.8424175
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