The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Rapid Assessment of Drug Susceptibilities of Mycobacterium tuberculosis by Means of Luciferase Reporter Phages
Abstract
Effective chemotherapy of tuberculosis requires rapid assessment of drug sensitivity because of the emergence of multidrug-resistant Mycobacterium tuberculosis . Drug susceptibility was assessed by a simple method based on the efficient production of photons by viable mycobacteria infected with specific reporter phages expressing the firefly luciferase gene. Light production was dependent on phage infection, expression of the luciferase gene, and the level of cellular adenosine triphosphate. Signals could be detected within minutes after infection of virulent M. tuberculosis with reporter phages. Culture of conventional strains with antituberculosis drugs, including isoniazid or rifampicin, resulted in extinction of light production. In contrast, light signals after luciferase reporter phage infection of drug-resistant strains continued to be produced. Luciferase reporter phages may help to reduce the time required for establishing antibiotic sensitivity of M. tuberculosis strains from weeks to days and to accelerate screening for new antituberculosis drugs.
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Citations
Cites
Lysogeny and transformation in mycobacteria: stable expression of foreign genes.
Snapper, S. B., Lugosi, L., Jekkel, A., Melton, R. E., Kieser, T., Bloom, B. R., & Jacobs, W. R. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.18.6987 · Cited by 326
Self-citationIntroduction of foreign DNA into mycobacteria using a shuttle phasmid
Jacobs, W. R., Tuckman, M., & Bloom, B. R. (1987) · Nature · doi:10.1038/327532a0 · Cited by 314
Self-citation
Cited by
Phage therapy: From biological mechanisms to future directions
Strathdee, S. A., Hatfull, G. F., Mutalik, V. K., & Schooley, R. T. (2023) · Cell · doi:10.1016/j.cell.2022.11.017 · Cited by 817
Self-citationFrieden, T. R., Sterling, T. R., Munsiff, S. S., Watt, C. J., & Dye, C. (2003) · The Lancet · doi:10.1016/s0140-6736(03)14333-4 · Cited by 689
Jordan, T. C., Burnett, S. H., Carson, S., Caruso, S. M., Clase, K., DeJong, R. J., Dennehy, J. J., Denver, D. R., Dunbar, D., Elgin, S. C. R., et al. (2014) · mBio · doi:10.1128/mbio.01051-13 · Cited by 509
Self-citationMycobacterium smegmatis: The Vanguard of Mycobacterial Research
Sparks, I. L., Derbyshire, K. M., Jacobs, W. R., & Morita, Y. S. (2023) · Journal of Bacteriology · doi:10.1128/jb.00337-22 · Cited by 149
Self-citationMshana, R. N., Tadesse, G., Abate, G., & Miörner, H. (1998) · Journal of Clinical Microbiology · doi:10.1128/jcm.36.5.1214-1219.1998 · Cited by 139
Metadata
- Authors
- Jacobs, W. R., Barletta, R. G., Udani, R., Chan, J., Kalkut, G., Sosne, G., Kieser, T., Sarkis, G. J., Hatfull, G. F., & Bloom, B. R.
- Venue
- Science
- Published
- 1993-05-07
- Volume
- 260
- Issue
- 5109
- Pages
- 819-822
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2117653437
- Cited by
- 439
- References
- 2
- FWCI
- 32.00
- Type
- journal article
- Language
- en
- Open access
- closed
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- Jacobs, W. R., Barletta, R. G., Udani, R., Chan, J., Kalkut, G., Sosne, G., Kieser, T., Sarkis, G. J., Hatfull, G. F., & Bloom, B. R. (1993). Rapid Assessment of Drug Susceptibilities of Mycobacterium tuberculosis by Means of Luciferase Reporter Phages. Science. https://doi.org/10.1126/science.8484123
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