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New Domain Motif: the Structure of Pectate Lyase C, a Secreted Plant Virulence Factor
Abstract
Pectate lyases are secreted by pathogens and initiate soft-rot diseases in plants by cleaving polygalacturonate, a major component of the plant cell wall. The three-dimensional structure of pectate lyase C from Erwinia chrysanthemi has been solved and refined to a resolution of 2.2 angstroms. The enzyme folds into a unique motif of parallel β strands coiled into a large helix. Within the core, the amino acids form linear stacks and include a novel asparagine ladder. The sequence similarities that pectate lyases share with pectin lyases, pollen and style proteins, and tubulins suggest that the parallel β helix motif may occur in a broad spectrum of proteins.
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Cited by
Wong, T. Y., Preston, L. A., & Schiller, N. L. (2000) · Annual Review of Microbiology · doi:10.1146/annurev.micro.54.1.289 · Cited by 669
EXTRACELLULAR ENZYMES AND PATHOGENESIS OF SOFT-ROT ERWINIA
Barras, F., van Gijsegem, F., & Chatterjee, A. K. (1994) · Annual Review of Phytopathology · doi:10.1146/annurev.py.32.090194.001221 · Cited by 533
The structure of bacterial outer membrane proteins
Schulz, G. E. (2002) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/s0005-2736(02)00577-1 · Cited by 486
Kisker, C., Schindelin, H., Alber, B. E., Ferry, J. G., & Rees, D. C. (1996) · The EMBO Journal · doi:10.1002/j.1460-2075.1996.tb00588.x · Cited by 259
Bioconversion of red seaweed galactans: a focus on bacterial agarases and carrageenases
Michel, G., Nyval-Collen, P., Barbeyron, T., Czjzek, M., & Helbert, W. (2006) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-006-0377-7 · Cited by 240
Metadata
- Authors
- Yoder, M. D., Keen, N. T., & Jurnak, F.
- Venue
- Science
- Published
- 1993-06-04
- Volume
- 260
- Issue
- 5113
- Pages
- 1503-1507
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2045476844
- Cited by
- 453
- References
- 0
- FWCI
- 33.86
- Type
- journal article
- Language
- en
- Open access
- closed
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- Yoder, M. D., Keen, N. T., & Jurnak, F. (1993). New Domain Motif: the Structure of Pectate Lyase C, a Secreted Plant Virulence Factor. Science. https://doi.org/10.1126/science.8502994
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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