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Plasma Membrane Vesiculation: A New Technique for Isolation of Plasma Membranes
Abstract
Monolayer cell cultures of macrophages, monocytes, myoblasts, and density-inhibited and transformed fibroblasts form and release cell surface membrane vesicles following exposure to formaldehyde, related low-molecular-weight aldehydes, and disulfide blocking agents. Vesicles have a unique composition of proteins and lipids. They show enrichment of cholesterol and sphingomyelin content and a seven-to tenfold enrichment of 5′-nucleotidase activity. Vesicles also contain intramembranous particles and show a trilamellar unit membrane and no ultrastructural evidence of contamination with other cytoplasmic organelles. The technique is proposed as a novel method for isolating plasma membrane vesicles from cells in culture.
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Cited by
Pegtel, D. M., & Gould, S. J. (2019) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-013118-111902 · Cited by 2,682
Elucidating membrane structure and protein behavior using giant plasma membrane vesicles
Sezgin, E., Kaiser, H.-J., Baumgart, T., Schwille, P., Simons, K., & Levental, I. (2012) · Nature Protocols · doi:10.1038/nprot.2012.059 · Cited by 527
Cooper, D. N., & Barondes, S. H. (1990) · The Journal of cell biology · doi:10.1083/jcb.110.5.1681 · Cited by 462
Sezgin, E., Levental, I., Grzybek, M., Schwarzmann, G., Mueller, V., Honigmann, A., Belov, V. N., Eggeling, C., Coskun, Ü., Simons, K., et al. (2012) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2012.03.007 · Cited by 334
A monoclonal antibody detaches embryonic skeletal muscle from extracellular matrices.
Neff, N. T., Lowrey, C., Decker, C., Tovar, A., Damsky, C., Buck, C., & Horwitz, A. F. (1982) · The Journal of cell biology · doi:10.1083/jcb.95.2.654 · Cited by 306
Metadata
- Authors
- Scott, R. E.
- Venue
- Science
- Published
- 1976-11-12
- Volume
- 194
- Issue
- 4266
- Pages
- 743-745
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2093861837
- Cited by
- 270
- References
- 0
- FWCI
- 5.65
- Type
- journal article
- Language
- en
- Open access
- closed
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- Scott, R. E. (1976). Plasma Membrane Vesiculation: A New Technique for Isolation of Plasma Membranes. Science. https://doi.org/10.1126/science.982044
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
