Zika virus produces noncoding RNAs using a multi-pseudoknot structure that confounds a cellular exonuclease
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Zika virus uses a convoluted RNA fold to produce noncoding RNAs associated with pathogenesis.
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Two stabilizing RNA elements (Zika virus-derived xrRNA1 dumbbell and evopreQ1 pseudoknot) led to a robust increase in bcgRNA UMI counts, while protein knockdown was indistinguishable among six structures.
“While protein knockdown was indistinguishable for all six structures (Fig. 5b,c and Extended Data Fig. 9b), we found that two elements (Zika virus-derived xrRNA1 dumbbell33; evopreQ1 pseudoknot34) led to a robust increase in bcgRNA unique molecular identifier (UMI) counts (Fig. 5d).”
From Efficient combinatorial targeting of RNA transcripts in single cells with Cas13 RNA Perturb-seq (2023) · cites this paper as [33]
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Parallel molecular evolution and adaptation in viruses
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Exoribonuclease-Resistant RNAs Exist within both Coding and Noncoding Subgenomic RNAs
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RNA stabilization by a poly(A) tail 3′-end binding pocket and other modes of poly(A)-RNA interaction
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Functional RNA structures throughout the Hepatitis C Virus genome
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Roadblocks and fast tracks: How RNA binding proteins affect the viral RNA journey in the cell
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RNA regulatory processes in RNA virus biology
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The exoribonuclease Xrn1 is a post-transcriptional negative regulator of autophagy
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Inhibition of type I interferon induction and signalling by mosquito-borne flaviviruses
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Long non‐coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution
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Different tertiary interactions create the same important 3D features in a distinct flavivirus xrRNA
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MicroRNAs 145 and 148a Are Upregulated During Congenital Zika Virus Infection
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- Authors
- Akiyama, B. M., Laurence, H. M., Massey, A. R., Costantino, D. A., Xie, X., Yang, Y., Shi, P.-Y., Nix, J. C., Beckham, J. D., & Kieft, J. S.
- Affiliations
- University of Colorado Anschutz (US)
- Howard Hughes Medical Institute (US)
- The University of Texas Medical Branch at Galveston (US)
- Lawrence Berkeley National Laboratory (US)
- Corresponding author
- Laurence, Hannah M.
- Massey, Aaron R.
- Kieft, Jeffrey S.
- ORCID
- Venue
- Science
- Published
- 2016-12-02
- Volume
- 354
- Issue
- 6316
- Pages
- 1148-1152
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- PMC
- PMC5476369
- OpenAlex
- W2550011929
- Cited by
- 245
- References
- 44
- Citations by year
- 2026: 21 · 2025: 15 · 2024: 16 · 2023: 18 · 2022: 14 · 2021: 38 · 2020: 35 · 2019: 29 · 2018: 28 · 2017: 29 · 2016: 1
- FWCI
- 28.76
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
- Funders
- HHMI–Burroughs Wellcome Fund
- University of Colorado School of Medicine and Department of Medicine
- Novartis Institutes for BioMedical Research
- University of Texas Medical Branch
- University of Texas STARs
- UC Cancer Center (P30CA046934)
- NIH (F32GM117730, R35GM118070, R01GM081346, R01AI087856, S10OD012033)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Mosquito-borne diseases and control
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Health Sciences › Medicine › Infectious Diseases › Viral Infections and Vectors
- Keywords
- Zika virus, exonuclease resistance, flavivirus pathogenesis, microcephaly, RNA secondary structure, mosquito cells
- MeSH terms
- Zika Virus Infection, Zika Virus, Animals, Chlorocebus aethiops, Epithelial Cells, Exoribonucleases, Humans, Culicidae, Mutation, Neurons, Nucleic Acid Conformation, RNA, Viral, Vero Cells, RNA, Untranslated, Mice
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