Cited by 245Mosquito-borne diseases and controlRNA and protein synthesis mechanismsViral Infections and Vectors

Zika virus produces noncoding RNAs using a multi-pseudoknot structure that confounds a cellular exonuclease

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Akiyama, Benjamin M., Laurence, Hannah M., Massey, Aaron R., Costantino, David A., Xie, Xuping, Yang, Yujiao, Shi, Pei-Yong, Nix, Jay C. +2 more·2016·Science

Abstract

Zika virus uses a convoluted RNA fold to produce noncoding RNAs associated with pathogenesis.

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Crossref · 10.1126/science.aah3963

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  • Two stabilizing RNA elements (Zika virus-derived xrRNA1 dumbbell and evopreQ1 pseudoknot) led to a robust increase in bcgRNA UMI counts, while protein knockdown was indistinguishable among six structures.

    “While protein knockdown was indistinguishable for all six structures (Fig. 5b,c and Extended Data Fig. 9b), we found that two elements (Zika virus-derived xrRNA1 dumbbell33; evopreQ1 pseudoknot34) led to a robust increase in bcgRNA unique molecular identifier (UMI) counts (Fig. 5d).”

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Research works printed but not linked: 5

  1. [34]Chang JH Xiang S Xiang K Manley JL Tong L Structural and biochemical studies of the 5′–>3′ exoribonuclease Xrn1 Nature structural & molecular biology 18 270 276 2011 PMC3075561 21297639
  2. [36]Chapman EG The structural basis of pathogenic subgenomic flavivirus RNA (sfRNA) production Science 344 307 310 2014 24744377 PMC4163914
  3. [38]Shan C An Infectious cDNA Clone of Zika Virus to Study Viral Virulence, Mosquito Transmission, and Antiviral Inhibitors Cell host & microbe 19 891 900 2016 27198478 PMC5206987
  4. [49]Pijlman GP A highly structured, nuclease-resistant, noncoding RNA produced by flaviviruses is required for pathogenicity Cell host & microbe 4 579 591 2008 19064258
  5. [50]Villordo SM Carballeda JM Filomatori CV Gamarnik AV RNA Structure Duplications and Flavivirus Host Adaptation Trends in microbiology 24 270 283 2016 26850219 PMC4808370

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Authors
Akiyama, B. M., Laurence, H. M., Massey, A. R., Costantino, D. A., Xie, X., Yang, Y., Shi, P.-Y., Nix, J. C., Beckham, J. D., & Kieft, J. S.
Affiliations
  • University of Colorado Anschutz (US)
  • Howard Hughes Medical Institute (US)
  • The University of Texas Medical Branch at Galveston (US)
  • Lawrence Berkeley National Laboratory (US)
Corresponding author
  • Laurence, Hannah M.
  • Massey, Aaron R.
  • Kieft, Jeffrey S.
Venue
Science
Published
2016-12-02
Volume
354
Issue
6316
Pages
1148-1152
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2550011929
Cited by
245
References
44
Citations by year
2026: 21 · 2025: 15 · 2024: 16 · 2023: 18 · 2022: 14 · 2021: 38 · 2020: 35 · 2019: 29 · 2018: 28 · 2017: 29 · 2016: 1
FWCI
28.76
Type
journal article
Language
en
Open access
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
Funders
  • HHMI–Burroughs Wellcome Fund
  • University of Colorado School of Medicine and Department of Medicine
  • Novartis Institutes for BioMedical Research
  • University of Texas Medical Branch
  • University of Texas STARs
  • UC Cancer Center (P30CA046934)
  • NIH (F32GM117730, R35GM118070, R01GM081346, R01AI087856, S10OD012033)
Topics
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Mosquito-borne diseases and control
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Health Sciences › Medicine › Infectious Diseases › Viral Infections and Vectors
Keywords
Zika virus, exonuclease resistance, flavivirus pathogenesis, microcephaly, RNA secondary structure, mosquito cells
MeSH terms
Zika Virus Infection, Zika Virus, Animals, Chlorocebus aethiops, Epithelial Cells, Exoribonucleases, Humans, Culicidae, Mutation, Neurons, Nucleic Acid Conformation, RNA, Viral, Vero Cells, RNA, Untranslated, Mice

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