The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Cortical column and whole-brain imaging with molecular contrast and nanoscale resolution
Open accessAbstract
Combining expansion and the lattice light sheet: Optical and electron microscopy have made tremendous inroads into understanding the complexity of the brain. Gao et al. introduce an approach for high-resolution tracing of neurons, their subassemblies, and their molecular constituents over large volumes. They applied their method, which combines expansion microscopy and lattice light-sheet microscopy, to the mouse cortical column and the entire Drosophila brain. The approach can be performed at speeds that should enable high-throughput comparative studies of neural development, circuit stereotypy, and structural correlations to neural activity or behavior. Science , this issue p. eaau8302
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Cites
Fiji: an open-source platform for biological-image analysis
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Ultrasensitive fluorescent proteins for imaging neuronal activity
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“5-kg organ that, despite its small size, contains more than 80 billion neurons (1) that connect through approximately 7000 synapses each in a network of immense complexity.”
BackgroundA GAL4-Driver Line Resource for Drosophila Neurobiology
Jenett, A., Rubin, G. M., Ngo, T.-T. B., Shepherd, D., Murphy, C., Dionne, H., Pfeiffer, B. D., Cavallaro, A., Hall, D., Jeter, J., et al. (2012) · Cell Reports · doi:10.1016/j.celrep.2012.09.011 · Cited by 1,449
Self-citationStructure and function of dendritic spines
Nimchinsky, E. A., Sabatini, B. L., & Svoboda, K. (2002) · Annual Review of Physiology · doi:10.1146/annurev.physiol.64.081501.160008 · Cited by 1,208
Long-term dendritic spine stability in the adult cortex
Grutzendler, J., Kasthuri, N., & Gan, W.-B. (2002) · Nature · doi:10.1038/nature01276 · Cited by 1,198 · cited 1×
“This is consistent with a longitudinal in vivo study of spine morphology that found that spines closer to the soma, including those on proximal apical dendrites, were more mature and formed stronger synaptic connections than those on basal dendrites or the distal apical dendrite (62).”
ResultMyelination and support of axonal integrity by glia
Nave, K.-A. (2010) · Nature · doi:10.1038/nature09614 · Cited by 1,062
A Complete Electron Microscopy Volume of the Brain of Adult Drosophila melanogaster
Zheng, Z., Lauritzen, J. S., Perlman, E., Robinson, C. G., Nichols, M., Milkie, D., Torrens, O., Price, J., Fisher, C. B., Sharifi, N., et al. (2018) · Cell · doi:10.1016/j.cell.2018.06.019 · Cited by 1,050
Self-citationMicheva, K. D., & Smith, S. J. (2007) · Neuron · doi:10.1016/j.neuron.2007.06.014 · Cited by 787
Cited by
Light sheet fluorescence microscopy
Stelzer, E. H. K., Strobl, F., Chang, B.-J., Preusser, F., Preibisch, S., McDole, K., & Fiolka, R. (2021) · Nature Reviews Methods Primers · doi:10.1038/s43586-021-00069-4 · Cited by 334
A hybrid erbium(III)–bacteriochlorin near-infrared probe for multiplexed biomedical imaging
Wang, T., Wang, S., Liu, Z., He, Z., Yu, P., Zhao, M., Zhang, H., Lu, L., Wang, Z., Wang, Z., et al. (2021) · Nature Materials · doi:10.1038/s41563-021-01063-7 · Cited by 308
The Functional Impact of Mitochondrial Structure Across Subcellular Scales
Glancy, B., Kim, Y., Katti, P., & Willingham, T. B. (2020) · Frontiers in Physiology · doi:10.3389/fphys.2020.541040 · Cited by 265
A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility
Sheu, S.-H., Upadhyayula, S., Dupuy, V., Pang, S., Deng, F., Wan, J., Walpita, D., Pasolli, H. A., Houser, J., Sanchez-Martinez, S., et al. (2022) · Cell · doi:10.1016/j.cell.2022.07.026 · Cited by 162
Self-citationDense neuronal reconstruction through X-ray holographic nano-tomography
Kuan, A. T., Phelps, J. S., Thomas, L. A., Nguyen, T. M., Han, J., Chen, C.-L., Azevedo, A. W., Tuthill, J. C., Funke, J., Cloetens, P., et al. (2020) · Nature Neuroscience · doi:10.1038/s41593-020-0704-9 · Cited by 160
Metadata
- Authors
- Gao, R., Asano, S. M., Upadhyayula, S., Pisarev, I., Milkie, D. E., Liu, T.-L., Singh, V., Graves, A., Huynh, G. H., Zhao, Y., Bogovic, J., Colonell, J., Ott, C. M., Zugates, C., Tappan, S., Rodriguez, A., Mosaliganti, K. R., Sheu, S.-H., Pasolli, H. A., Pang, S., Xu, C. S., Megason, S. G., Hess, H., Lippincott-Schwartz, J., Hantman, A., Rubin, G. M., Kirchhausen, T., Saalfeld, S., Aso, Y., Boyden, E. S., & Betzig, E.
- Venue
- Science
- Published
- 2019-01-18
- Volume
- 363
- Issue
- 6424
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2910779362
- Cited by
- 370
- References
- 27
- FWCI
- 27.29
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Gao, R., Asano, S. M., Upadhyayula, S., Pisarev, I., Milkie, D. E., Liu, T.-L., Singh, V., Graves, A., Huynh, G. H., Zhao, Y., Bogovic, J., Colonell, J., Ott, C. M., Zugates, C., Tappan, S., Rodriguez, A., Mosaliganti, K. R., Sheu, S.-H., Pasolli, H. A., . . . Betzig, E. (2019). Cortical column and whole-brain imaging with molecular contrast and nanoscale resolution. Science. https://doi.org/10.1126/science.aau8302
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
