The DOI matches its Crossref record.
Design of an in vitro biocatalytic cascade for the manufacture of islatravir
Abstract
Maximal efficiency from enzyme cascades: Enzymes are highly selective catalysts that can be useful for specific transformations in organic synthesis. Huffman et al. combined designer enzymes in a multistep cascade reaction (see the Perspective by O'Reilly and Ryan). The approach eliminates purification steps, recycles expensive cofactors, and couples favorable and unfavorable reactions. With the target molecule islatravir, an experimental HIV drug, they optimized five enzymes by directed evolution to be compatible with unnatural substrates and stable in the reaction conditions. Stereochemical purity was amplified at every enzymatic step, and the final synthesis was both atom economical and efficient. Science , this issue p. 1255 ; see also p. 1199
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Cited by
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Goldman, S., Das, R., Yang, K. K., & Coley, C. W. (2022) · PLOS Computational Biology · doi:10.1371/journal.pcbi.1009853 · Cited by 120
Selective Phosphorylation of Phenols and Benzenediols by the Kinase PsiK and Variants Thereof
Kim, A., Morato, N. M., Saha, P., Eyimegwu, P. N., Niyaz, A. A., Huang, R., Cooks, R. G., Phelan, R. M., & Lewis, J. C. (2025) · Angewandte Chemie International Edition · doi:10.1002/anie.202503538 · Cited by 7
Accessing Active Fragments for Drug Discovery Utilising Nitroreductase Biocatalysis
Holder, L., Yuce, E., Oriomah, G., Jenkins, A., Reynisson, J., Winter, A., & Cosgrove, S. C. (2024) · ChemBioChem · doi:10.1002/cbic.202400428 · Cited by 2
Metadata
- Authors
- Huffman, M. A., Fryszkowska, A., Alvizo, O., Borra-Garske, M., Campos, K. R., Canada, K. A., Devine, P. N., Duan, D., Forstater, J. H., Grosser, S. T., Halsey, H. M., Hughes, G. J., Jo, J., Joyce, L. A., Kolev, J. N., Liang, J., Maloney, K. M., Mann, B. F., Marshall, N. M., McLaughlin, M., Moore, J. C., Murphy, G. S., Nawrat, C. C., Nazor, J., Novick, S., Patel, N. R., Rodriguez-Granillo, A., Robaire, S. A., Sherer, E. C., Truppo, M. D., Whittaker, A. M., Verma, D., Xiao, L., Xu, Y., & Yang, H.
- Venue
- Science
- Published
- 2019-12-06
- Volume
- 366
- Issue
- 6470
- Pages
- 1255-1259
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2991758999
- Cited by
- 641
- References
- 0
- FWCI
- 20.62
- Type
- journal article
- Language
- en
- Open access
- closed
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