The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Mechanisms of OCT4-SOX2 motif readout on nucleosomes
Abstract
Engaging the nucleosome: Cell identity is defined by gene expression patterns that are established through the binding of specific transcription factors. However, nucleosomal units limit access of transcription factors to specific DNA motifs within the mammalian genome. To study how transcription factors bind such chromatinized, nucleosome-embedded motifs, Michael et al. focused on the pluripotency factors OCT4 and SOX2. They systematically quantified the relative affinities of these factors at different motif positions throughout the nucleosome, enabling structure determination of OCT4-SOX2–bound nucleosomes by cryo–electron microscopy. OCT4 and SOX2 bound cooperatively to strengthen DNA-binding affinity and resulted in DNA distortions that destabilized the nucleosome. This analysis reveals position-dependent binding modes that were validated in vivo, providing insights on how transcription factors read out chromatinized motifs. Science , this issue p. 1460
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Nucleosome binding by the pioneer transcription factor OCT4
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Metadata
- Authors
- Michael, A. K., Grand, R. S., Isbel, L., Cavadini, S., Kozicka, Z., Kempf, G., Bunker, R. D., Schenk, A. D., Graff-Meyer, A., Pathare, G. R., Weiss, J., Matsumoto, S., Burger, L., Schübeler, D., & Thomä, N. H.
- Venue
- Science
- Published
- 2020-06-26
- Volume
- 368
- Issue
- 6498
- Pages
- 1460-1465
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W3019281170
- Cited by
- 235
- References
- 10
- FWCI
- 9.27
- Type
- journal article
- Language
- en
- Open access
- closed
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- Michael, A. K., Grand, R. S., Isbel, L., Cavadini, S., Kozicka, Z., Kempf, G., Bunker, R. D., Schenk, A. D., Graff-Meyer, A., Pathare, G. R., Weiss, J., Matsumoto, S., Burger, L., Schübeler, D., & Thomä, N. H. (2020). Mechanisms of OCT4-SOX2 motif readout on nucleosomes. Science. https://doi.org/10.1126/science.abb0074
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