Absence of Amphotericin B-Tolerant Persister Cells in Biofilms of Some Candida Species
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Biofilms and planktonic cells of five Candida species were surveyed for the presence of persister (drug-tolerant) cell populations after exposure to amphotericin B. None of the planktonic cultures (exponential or stationary phase) contained persister cells. However, persisters were found in biofilms of one of two strains of Candida albicans tested and in biofilms of Candida krusei and Candida parapsilosis , but not in biofilms of Candida glabrata or Candida tropicalis . These results suggest that persister cells cannot solely account for drug resistance in Candida biofilms.
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Antifungal persistence is not a common phenomenon.
“suggesting that antifungal persistence is not a common phenomenon [79,82].”
From Antifungal persistence: Clinical relevance and mechanisms (2025) · cites this paper as [82]
The survey summary (p. 1) reports no persisters in the tested planktonic Candida cultures and persisters in only some of the tested biofilms. That supports persistence not being universal in these amphotericin B experiments, but it does not establish that antifungal persistence is uncommon across fungi or antifungal treatments. (LLM, conf=0.85)
Experimental — accuracy not yet measuredAmB-tolerant persister cells are absent in biofilms of some Candida spp.
“Notably, AmB-tolerant persister cells are absent in biofilms of some Candida spp., suggesting that antifungal persistence is not a common phenomenon [79,82].”
From Antifungal persistence: Clinical relevance and mechanisms (2025) · cites this paper as [82]
The presence of persister cells in biofilms is mainly responsible for biofilm resistance.
“Al-Dhaheri and Douglas [46] found that the presence of persister cells in biofilms is mainly responsible for biofilm resistance.”
From Candida glabrata: pathogenicity and resistance mechanisms for adaptation and survival (2021) · cites this paper as [46]
Such a persister population was absent from biofilms of C. glabrata.
“Such a ‘persister’ population was absent from the biofilms of C. glabrata.”
From Candida glabrata: pathogenicity and resistance mechanisms for adaptation and survival (2021) · cites this paper as [46]
Al-Dhaheri and Douglas reported that persister populations were observed in biofilms of C. albicans, C. krusei, and C. parapsilosis after exposure to amphotericin.
“The findings agreed with the study reported by Al-Dhaheri and Douglas [46] that ‘persister’ populations were observed in biofilms of C. albicans, C. krusei, and C. parapsilosis after exposure to amphotericin.”
From Candida glabrata: pathogenicity and resistance mechanisms for adaptation and survival (2021) · cites this paper as [46]
Yeast persister cells were first discovered as a small population in C. albicans biofilms.
“Yeast persister cells were first discovered as a small population in C. albicans biofilms [165,166].”
From Candida glabrata Biofilms: How Far Have We Come? (2017) · cites this paper as [166]
Al-Dhaheri and Douglas reported persister cells in biofilms treated with amphotericin B from isolates of Candida species.
“Al-Dhaheri and Douglas [166] reported persister cells in biofilms treated with amphotericin B from isolates of Candida species.”
From Candida glabrata Biofilms: How Far Have We Come? (2017) · cites this paper as [166]
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References 23 · Cited by 99
- Authors
- Al-Dhaheri, R. S., & Douglas, L. J.
- Affiliations
- University of Glasgow (GB)
- Corresponding author
- Douglas, L. Julia
- ORCID
- Douglas, L. Julia
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2008-05
- Volume
- 52
- Issue
- 5
- Pages
- 1884-1887
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- PMC
- PMC2346666
- OpenAlex
- W2138412094
- Cited by
- 99
- References
- 23
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 4 · 2023: 6 · 2022: 4 · 2021: 8 · 2020: 5 · 2019: 4 · 2018: 3 · 2017: 5 · 2016: 3 · 2015: 9 · 2014: 2 · 2013: 3 · 2012: 4
- FWCI
- 6.68
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Antimicrobial Agents and Chemotherapy (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Keywords
- persister cells, Candida biofilms, Candida albicans, Candida krusei, Candida parapsilosis, Candida glabrata, Candida tropicalis, antifungal resistance, planktonic cells, fungal biofilms
- MeSH terms
- Amphotericin B, Antifungal Agents, Candida, Species Specificity, Biofilms, Drug Resistance, Fungal
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