The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
BOCILLIN FL, a Sensitive and Commercially Available Reagent for Detection of Penicillin-Binding Proteins
Open accessAbstract
We describe a new, sensitive, rapid, and nonradioactive method involving the use of the commercially available BOCILLIN FL, a fluorescent penicillin, as a labeling reagent for the detection and study of penicillin-binding proteins (PBPs). This method allowed rapid detection of 30 ng of a purified PBP protein under UV light and of 2 to 4 ng of the protein with the aid of a FluorImager. This method also allowed rapid determination of the PBP profiles of Escherichia coli , Pseudomonas aeruginosa , and Streptococcus pneumoniae . The PBP profiles obtained are virtually identical to those reported previously with 3 H-, 14 C-, or 125 I-labeled penicillin. Using this method enabled us to determine the 50% inhibitory concentrations of the penicillin-sensitive and -resistant PBP2x proteins of S. pneumoniae for penicillin G, thereby allowing a direct evaluation of their relative affinities for penicillin G. Finally, this method also allowed us to compare relative affinities of a PBP2x protein for different β-lactam antibiotics with the aid of fluorescence polarization technology and to monitor a PBP2x protein during purification.
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Citations
Cites
The crisis in antibiotic resistance
Neu, H. C. (1992) · Science · doi:10.1126/science.257.5073.1064 · Cited by 2,834 · cited 3×
“Resistance to b-lactam antibiotics in some species such as Streptococcus pneumoniae has occurred by development of altered high-molecular-mass PBPs which have reduced affinity for the antibiotics (6, 7, 10, 11, 14, 24, 25, 29, 30).”
Background
Cited by
Bacterial Resistance to β-Lactam Antibiotics: Compelling Opportunism, Compelling Opportunity
Fisher, J. F., Meroueh, S. O., & Mobashery, S. (2005) · Chemical Reviews · doi:10.1021/cr030102i · Cited by 913
Pogliano, J., Pogliano, N., & Silverman, J. A. (2012) · Journal of Bacteriology · doi:10.1128/jb.00011-12 · Cited by 319
Positive role of peptidoglycan breaks in lactococcal biofilm formation
Mercier, C., Durrieu, C., Briandet, R., Domakova, E., Tremblay, J., Buist, G., & Kulakauskas, S. (2002) · Molecular Microbiology · doi:10.1046/j.1365-2958.2002.03160.x · Cited by 88
Akusobi, C., Benghomari, B. S., Zhu, J., Wolf, I. D., Singhvi, S., Dulberger, C. L., Ioerger, T. R., & Rubin, E. J. (2022) · eLife · doi:10.7554/elife.71947 · Cited by 45
“Therefore, to test whether PBP- lipo is an active PBP in Mab, we determined whether PBPlipo binds bocillin FL, a fluorescent analog of penicillin (Zhao et al., 1999).”
Background
Metadata
- Authors
- Zhao, G., Meier, T. I., Kahl, S. D., Gee, K. R., & Blaszczak, L. C.
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 1999-05
- Volume
- 43
- Issue
- 5
- Pages
- 1124-1128
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- OpenAlex
- W2114533737
- Cited by
- 300
- References
- 1
- FWCI
- 1.86
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: BOCILLIN FL, a Sensitive and Commercially Available Reagent for Detection of Penicillin-Binding Proteins. https://app.cochat.ai/papers/10.1128/aac.43.5.1124
- The paper
- Zhao, G., Meier, T. I., Kahl, S. D., Gee, K. R., & Blaszczak, L. C. (1999). BOCILLIN FL, a Sensitive and Commercially Available Reagent for Detection of Penicillin-Binding Proteins. Antimicrobial Agents and Chemotherapy. https://doi.org/10.1128/aac.43.5.1124
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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