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Cited by 94Antibiotic Resistance in BacteriaEscherichia coli research studiesPharmaceutical and Antibiotic Environmental Impacts

Detection of CTX-M-Type β-Lactamase Genes in Fecal Escherichia coli Isolates from Healthy Children in Bolivia and Peru

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Pallecchi, Lucia, Malossi, Monica, Mantella, Antonia, Gotuzzo, Eduardo, Trigoso, Christian, Bartoloni, Alessandro, Paradisi, Franco, Kronvall, Göran +1 more·2004·Antimicrobial Agents and Chemotherapy
DOI 10.1128/aac.48.12.4556-4561.2004PMC PMC529195
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Abstract

A survey was carried out from August to November 2002 to evaluate the antimicrobial susceptibilities of fecal Escherichia coli isolates from 3,208 healthy children from four different urban areas of Latin America, two in Bolivia (Camiri and Villa Montes) and two in Peru (Yurimaguas and Moyobamba). Ceftriaxone-resistant E. coli isolates were detected in four children, one from each of the areas sampled. The isolates exhibited a multidrug-resistant phenotype, including resistance to oxyimino-cephalosporins and aztreonam, and the MICs of ceftazidime for the isolates were lower than those of cefotaxime. By PCR and sequencing, the bla CTX-M-2 determinant was detected in three isolates and the bla CTX-M-15 determinant was detected in one isolate (from Peru). The CTX-M-2-producing isolates belonged to three different phylogenetic groups (groups A, B2, and D), while the CTX-M-15-producing isolate belonged to phylogenetic group D. The bla CTX-M-2 determinants were transferable to E. coli by conjugation, while conjugative transfer of the bla CTX-M-15 determinant was not detectable. Plasmids harboring the bla CTX-M-2 determinant exhibited similar restriction profiles, and in all of them the gene was located on a 2.2-kb PstI fragment, suggesting a genetic environment similar to that present in In35 and InS21. The findings of the present study confirm the widespread distribution of CTX-M-type β-lactamases and underscore the role that commensal E. coli isolates could play as a potential reservoir of these clinically relevant resistance determinants. This is the first report of CTX-M-type enzymes in Bolivia and Peru and also the first report of the detection of CTX-M-15 in Latin America.

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  • The molecular epidemiology of ESBL-E enzymes identified in the Madagascar, Nigeria, Cameroon, and Mozambique studies was in keeping with the global predominance of the CTX-M clone.

    “The molecular epidemiology of the ESBL-E enzymes identified in the Madagascar (Chereau et al., 2015), Nigeria (Fortini et al., 2015), Cameroon (Djuikoue et al., 2016b), and Mozambique (Sáez-lópez et al., 2016) studies was in keeping with the global predominance of the CTX-M clone, which is also widely reported in community-acquired UTI (Pitout et al., 2005;Peirano and Pitout, 2010;Pallecchi et al.”

    From Maternal colonization or infection with extended-spectrum beta-lactamase-producing Enterobacteriaceae in Africa: A systematic review and meta-analysis (2017) · cites this paper as Pallecchi et al., 2004

    The Abstract (page unavailable) says CTX-M-type enzymes have been reported in clinical isolates from community-acquired infections, and the Discussion describes them as important emerging ESBLs worldwide. But the passage does not identify those infections as UTIs or establish that CTX-M is widely reported specifically in community-acquired UTI. The cited paper studied fecal isolates from healthy children, not UTI cases. (LLM, conf=0.84)

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  1. Sambrook J. and D. W. Russel. 2001. Molecular cloning: a laboratory manual 3rd ed. Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y.

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Authors
Pallecchi, L., Malossi, M., Mantella, A., Gotuzzo, E., Trigoso, C., Bartoloni, A., Paradisi, F., Kronvall, G., & Rossolini, G. M.
Affiliations
  • University of Siena (IT)
  • University of Florence (IT)
  • Universidad Peruana Cayetano Heredia (PE)
  • Ministerio de Salud (BO)
  • Department of Microbiology and Tumor Biology-MTC, Clinical Microbiology, Karolinska Institute, Karolinska Hospital L2:02, Stockholm, Sweden
  • Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, Siena
ORCID
Venue
Antimicrobial Agents and Chemotherapy
Publisher
American Society for Microbiology
Published
2004-12
Volume
48
Issue
12
Pages
4556-4561
ISSN
  • 0066-4804 (ISSN-L)
  • 1098-6596
DOI
10.1128/aac.48.12.4556-4561.2004
PMC
PMC529195
OpenAlex
W2096357623
Semantic Scholar
f75274dcd2972737568f1b1257cbec548194f561
Cited by
94
References
38
Citations by year
2025: 3 · 2024: 2 · 2023: 1 · 2022: 5 · 2021: 3 · 2020: 2 · 2019: 4 · 2018: 2 · 2017: 3 · 2015: 4 · 2014: 9 · 2013: 10 · 2012: 7
FWCI
2.69
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Antimicrobial Agents and Chemotherapy (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Florence Research (University of Florence) (submittedVersion)
  • Use Siena air (University of Siena) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
  • Physical Sciences › Environmental Science › Pollution › Pharmaceutical and Antibiotic Environmental Impacts
Keywords
CTX-M, CTX-M-2, CTX-M-15, Escherichia coli, children, antimicrobial resistance, ceftriaxone resistance, multidrug resistance, conjugative transfer, plasmids, phylogenetic groups, Latin America, Bolivia, Peru
MeSH terms
beta-Lactamases, Bolivia, Ceftriaxone, Cephalosporins, Conjugation, Genetic, DNA, Bacterial, Escherichia coli, Feces, Microbial Sensitivity Tests, Peru, Phylogeny, Reverse Transcriptase Polymerase Chain Reaction, Gene Transfer, Horizontal, Drug Resistance, Bacterial

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