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Possible Environmental Origin of Resistance of Aspergillus fumigatus to Medical Triazoles
Open accessAbstract
We reported the emergence of resistance to medical triazoles of Aspergillus fumigatus isolates from patients with invasive aspergillosis. A dominant resistance mechanism was found, and we hypothesized that azole resistance might develop through azole exposure in the environment rather than in azole-treated patients. We investigated if A. fumigatus isolates resistant to medical triazoles are present in our environment by sampling the hospital indoor environment and soil from the outdoor environment. Antifungal susceptibility, resistance mechanisms, and genetic relatedness were compared with those of azole-resistant clinical isolates collected in a previous study. Itraconazole-resistant A. fumigatus (five isolates) was cultured from the indoor hospital environment as well as from soil obtained from flower beds in proximity to the hospital (six isolates) but never from natural soil. Additional samples of commercial compost, leaves, and seeds obtained from a garden center and a plant nursery were also positive (four isolates). Cross-resistance was observed for voriconazole, posaconazole, and the azole fungicides metconazole and tebuconazole. Molecular analysis showed the presence of the dominant resistance mechanism, which was identical to that found in clinical isolates, in 13 of 15 environmental isolates, and it showed that environmental and clinical isolates were genetically clustered apart from nonresistant isolates. Patients with azole-resistant aspergillosis might have been colonized with azole-resistant isolates from the environment.
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“The Aspergillus species [4,5,6,7,8,9,10,11,12,13] are common fungi found in the environment, such as soil and air [14,15,16,17,18,19].”
From Nationwide Survey about the Occurrence of Aspergillosis in Captive Penguins in Zoos and Aquariums in Japan (2023) · cites this paper as [15]
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National Committee for Clinical Laboratory Standards
Bruck, E. (1980) · PEDIATRICS · doi:10.1542/peds.65.1.187 · Cited by 14,736
Glass, N. L., & Donaldson, G. C. (1995) · Applied and Environmental Microbiology · doi:10.1128/aem.61.4.1323-1330.1995 · Cited by 4,765
Emergence of Azole Resistance in Aspergillus fumigatus and Spread of a Single Resistance Mechanism
Snelders, E., van der Lee, H. A. L., Kuijpers, J., Rijs, A. J. M. M., Varga, J., Samson, R. A., Mellado, E., Donders, A. R. T., Melchers, W. J. G., & Verweij, P. E. (2008) · PLoS Medicine · doi:10.1371/journal.pmed.0050219 · Cited by 726
Self-citationMellado, E., Garcia-Effron, G., Alcázar-Fuoli, L., Melchers, W. J. G., Verweij, P. E., Cuenca-Estrella, M., & Rodríguez-Tudela, J. L. (2007) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01092-06 · Cited by 490
Self-citationMultiple-Triazole–Resistant Aspergillosis
Verweij, P. E., Mellado, E., & Melchers, W. J. G. (2007) · New England Journal of Medicine · doi:10.1056/nejmc061720 · Cited by 380
Self-citationEvolution of antifungal-drug resistance: mechanisms and pathogen fitness
Anderson, J. B. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1179 · Cited by 375
Diaz-Guerra, T. M., Mellado, E., Cuenca-Estrella, M., & Rodriguez-Tudela, J. L. (2003) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.47.3.1120-1124.2003 · Cited by 281
Polyphasic taxonomy of Aspergillus section Fumigati and its teleomorph Neosartorya
Samson, R. A., Hong, S., Peterson, S. W., Frisvad, J. C., & Varga, J. (2007) · Studies in Mycology · doi:10.3114/sim.2007.59.14 · Cited by 259
de Valk, H. A., Meis, J. F. G. M., Curfs-Breuker, I., Muehlethaler, K., Mouton, J. W., & Klaassen, C. H. W. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.8.4112-4120.2005 · Cited by 248
Tang, C. M., Cohen, J., & Holden, D. W. (1992) · Molecular Microbiology · doi:10.1111/j.1365-2958.1992.tb00891.x · Cited by 149
Ferreira, M. E. D. S., Capellaro, J. L., Marques, E. D. R., Malavazi, I., Perlin, D. S., Park, S., Anderson, J. B., Colombo, A. L., Arthington, B. A., Goldman, M. H. S., et al. (2004) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.48.11.4405-4413.2004 · Cited by 147
Cools, H. J., & FRAAIJE, B. A. (2008) · Pest Management Science · doi:10.1002/ps.1568 · Cited by 140
Bromilow, R. H., Evans, A. A., & Nicholls, P. H. (1999) · Pesticide Science · doi:10.1002/(sici)1096-9063(199912)55:12 · Cited by 116
Verweij, P. E., Dorsthorst, D. T. A. T., Rijs, A. J. M. M., de Vries-Hospers, H. G., & Meis, J. F. G. M. (2002) · Journal of Clinical Microbiology · doi:10.1128/jcm.40.7.2648-2650.2002 · Cited by 108
Self-citationNew taxa of Neosartorya and Aspergillus in Aspergillus section Fumigati
Hong, S., Shin, H., Hong, J., Frisvad, J. C., Nielsen, P. V., Varga, J., & Samson, R. A. (2007) · Antonie van Leeuwenhoek · doi:10.1007/s10482-007-9183-1 · Cited by 91
Azole‐Resistant Central Nervous System Aspergillosis
van der Linden, J. W. M., Jansen, R. R., Bresters, D., Visser, C. E., Geerlings, S. E., Kuijper, E. J., Melchers, W. J. G., & Verweij, P. E. (2009) · Clinical Infectious Diseases · doi:10.1086/597465 · Cited by 84
Self-citationBalajee, S. A., Tay, S. T., Lasker, B. A., Hurst, S. F., & Rooney, A. P. (2007) · Eukaryotic Cell · doi:10.1128/ec.00164-07 · Cited by 62
Hof, H. (2008) · Drug Resistance Updates · doi:10.1016/j.drup.2008.01.001 · Cited by 60
Serfling, A., Wohlrab, J., & Deising, H. B. (2007) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00654-07 · Cited by 48
Aspergillosis—and a misleading sensitivity result
van Leer‐Buter, C., Takes, R. P., Hebeda, K. M., Melchers, W. J. G., & Verweij, P. E. (2007) · The Lancet · doi:10.1016/s0140-6736(07)61055-1 · Cited by 38
Self-citationPersistence of hexaconazole, a triazole fungicide in soils
Singh, N., & Dureja, P. (2000) · Journal of Environmental Science and Health Part B · doi:10.1080/03601230009373291 · Cited by 21
Occurrence of dicarboximidic fungicides and their metabolites? residues in commercial compost
Vanni, A., Fontana, F., Gamberini, R., & Calabria, A. (2004) · Agronomie · doi:10.1051/agro:2003058 · Cited by 5
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1 of the 23 references couldn’t be matched to a known paper.
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- Bromilow, R. H., A. A. Evans, and P. H. Nicholls. 1999. Factors affecting degradation rates of five triazole fungicides in two soil types 1. Laboratory incubations. Pestic. Sci.55:1129-1134.
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Tackling the emerging threat of antifungal resistance to human health
Fisher, M. C., Alastruey-Izquierdo, A., Berman, J., Bicanic, T., Bignell, E. M., Bowyer, P., Bromley, M., Brüggemann, R., Garber, G., Cornely, O. A., et al. (2022) · Nature Reviews Microbiology · doi:10.1038/s41579-022-00720-1 · Cited by 1,179
Self-citationOverview of the Antimicrobial Compounds Produced by Members of the Bacillus subtilis Group
Caulier, S., Nannan, C., Gillis, A., Licciardi, F., Bragard, C., & Mahillon, J. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.00302 · Cited by 882
Azole resistance in Aspergillus fumigatus: a side-effect of environmental fungicide use?
Verweij, P. E., Snelders, E., Kema, G. H., Mellado, E., & Melchers, W. J. (2009) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(09)70265-8 · Cited by 604
Self-citationRegulatory circuitry governing fungal development, drug resistance, and disease.
Shapiro, R. S., Robbins, N., & Cowen, L. E. (2011) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00045-10 · Cited by 576
Antifungal drug resistance: evolution, mechanisms and impact
Revie, N. M., Iyer, K. R., Robbins, N., & Cowen, L. E. (2018) · Current Opinion in Microbiology · doi:10.1016/j.mib.2018.02.005 · Cited by 552
Metadata
- Authors
- Snelders, E., Huis in 't Veld, R. A. G., Rijs, A. J. M. M., Kema, G. H. J., Melchers, W. J. G., & Verweij, P. E.
- Affiliations
- Radboud University Nijmegen (NL)
- Department of Medical Microbiology
- Wageningen University & Research (NL)
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2009-06-15
- Volume
- 75
- Issue
- 12
- Pages
- 4053-4057
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC2698372
- OpenAlex
- W2146843443
- Cited by
- 423
- References
- 23
- Citations by year
- 2026: 15 · 2025: 21 · 2024: 28 · 2023: 18 · 2022: 13 · 2021: 40 · 2020: 23 · 2019: 25 · 2018: 36 · 2017: 40 · 2016: 27 · 2015: 29 · 2014: 21 · 2013: 19 · 2012: 24
- FWCI
- 15.64
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Socio-Environmental Systems Modeling (acceptedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Pure Amsterdam UMC (submittedVersion)
- Radboud Repository (Radboud University) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
- Funders
- ZonMw
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Fungal Plant Pathogen Control
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Aspergillus fumigatus, azole resistance, itraconazole, invasive aspergillosis, hospital indoor environment, soil, cross-resistance, metconazole, tebuconazole, voriconazole, posaconazole, antifungal susceptibility, genetic relatedness, environmental colonization
- MeSH terms
- Antifungal Agents, Aspergillosis, Aspergillus fumigatus, Cross Infection, DNA, Fungal, Environmental Microbiology, Genotype, Humans, Microbial Sensitivity Tests, Triazoles, Cluster Analysis, DNA Fingerprinting, Mycological Typing Techniques, Molecular Epidemiology, Microsatellite Repeats, Drug Resistance, Fungal
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