The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Conservation of the Chitin Utilization Pathway in the Vibrionaceae
Open accessAbstract
Vibrionaceae are regarded as important marine chitin degraders, and attachment to chitin regulates important biological functions; yet, the degree of chitin pathway conservation in Vibrionaceae is unknown. Here, a core chitin degradation pathway is proposed based on comparison of 19 Vibrio and Photobacterium genomes with a detailed metabolic map assembled for V. cholerae from published biochemical, genomic, and transcriptomic results. Further, to assess whether chitin degradation is a conserved property of Vibrionaceae, a set of 54 strains from 32 taxa were tested for the ability to grow on various forms of chitin. All strains grew on N-acetylglucosamine (GlcNAc), the monomer of chitin. The majority of isolates grew on alpha (crab shell) and beta (squid pen) chitin and contained chitinase A (chiA) genes. chiA sequencing and phylogenetic analysis suggest that this gene is a good indicator of chitin metabolism but appears subject to horizontal gene transfer and duplication. Overall, chitin metabolism appears to be a core function of Vibrionaceae, but individual pathway components exhibit dynamic evolutionary histories.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Vibrios are also known to express genes involved in the metabolic degradation of chitin (Hunt et al., 2008), an important component of the exoskeleton of the nauplii.”
From Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp Litopenaeus stylirostris : possible influence of the rearing water and more (2021) · cites this paper as Hunt et al., 2008
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Basic local alignment search tool
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990) · Journal of Molecular Biology · doi:10.1006/jmbi.1990.9999 · Cited by 98,669
A Simple, Fast, and Accurate Algorithm to Estimate Large Phylogenies by Maximum Likelihood
Guindon, S., & Gascuel, O. (2003) · Systematic Biology · doi:10.1080/10635150390235520 · Cited by 16,923
MUSCLE: a multiple sequence alignment method with reduced time and space complexity
Edgar, R. C. (2004) · BMC Bioinformatics · doi:10.1186/1471-2105-5-113 · Cited by 9,119
OrthoMCL: Identification of Ortholog Groups for Eukaryotic Genomes
Li, L., Stoeckert, C. J., & Roos, D. S. (2003) · Genome Research · doi:10.1101/gr.1224503 · Cited by 5,707
DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae
Heidelberg, J. F., Eisen, J. A., Nelson, W. C., Clayton, R. A., Gwinn, M. L., Dodson, R. J., Haft, D. H., Hickey, E. K., Peterson, J. D., Umayam, L. A., et al. (2000) · Nature · doi:10.1038/35020000 · Cited by 1,871
Global Climate and Infectious Disease: The Cholera Paradigm
Colwell, R. R. (1996) · Science · doi:10.1126/science.274.5295.2025 · Cited by 1,296
MEDIA FOR THE CULTURE OF OCEANIC ULTRAPHYTOPLANKTON 1,2
Keller, M. D., Selvin, R., Claus, W., & Guillard, R. R. L. (1987) · Journal of Phycology · doi:10.1111/j.1529-8817.1987.tb04217.x · Cited by 1,038
Chitin Induces Natural Competence in Vibrio cholerae
Meibom, K. L., Blokesch, M., Dolganov, N. A., Wu, C.-Y., & Schoolnik, G. K. (2005) · Science · doi:10.1126/science.1120096 · Cited by 623
The Vibrio cholerae chitin utilization program
Meibom, K. L., Li, X. B., Nielsen, A. T., Wu, C.-Y., Roseman, S., & Schoolnik, G. K. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0308707101 · Cited by 555
Phylogeny and Molecular Identification of Vibrios on the Basis of Multilocus Sequence Analysis
Thompson, F. L., Gevers, D., Thompson, C. C., Dawyndt, P., Naser, S., Hoste, B., Munn, C. B., & Swings, J. (2005) · Applied and Environmental Microbiology · doi:10.1128/aem.71.9.5107-5115.2005 · Cited by 487
Genotypic Diversity Within a Natural Coastal Bacterioplankton Population
Thompson, J. R., Preheim, S. P., Pharino, C., Klepac‐Ceraj, V., Hunt, D. E., Benoit, J., Sarma-Rupavtarm, R. B., Distel, D. L., & Polz, M. F. (2005) · Science · doi:10.1126/science.1106028 · Cited by 360
A colonization factor links Vibrio cholerae environmental survival and human infection
Kirn, T. J., Jude, B. A., & Taylor, R. K. (2005) · Nature · doi:10.1038/nature04249 · Cited by 357
Diversity and Dynamics of a North Atlantic Coastal Vibrio Community
Thompson, J. R., Randa, M. A., Marcelino, L. A., Tomita‐Mitchell, A., Lim, E. L., & Polz, M. F. (2004) · Applied and Environmental Microbiology · doi:10.1128/aem.70.7.4103-4110.2004 · Cited by 347
Physiological aspects of chitin catabolism in marine bacteria
Keyhani, N. O., & Roseman, S. (1999) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/s0304-4165(99)00172-5 · Cited by 328
Critical Factors Influencing the Occurrence of Vibrio cholerae in the Environment of Bangladesh
Huq, A., Sack, R. B., Nizam, A., Longini, I. M., Nair, G. B., Ali, A., Morris, J. G., Khan, M., Siddique, A., Yunus, M., et al. (2005) · Applied and Environmental Microbiology · doi:10.1128/aem.71.8.4645-4654.2005 · Cited by 328
Chemical composition of dissolved organic nitrogen in the ocean
McCarthy, M. D., Pratum, T. K., Hedges, J. I., & Benner, R. (1997) · Nature · doi:10.1038/36535 · Cited by 305
Dissimilatory arsenate and sulfate reduction in Desulfotomaculum auripigmentum sp. nov.
Newman, D. K., Kennedy, E. K., Coates, J. D., Ahmann, D., Ellis, D. J., Lovley, D. R., & Morel, F. M. M. (1997) · Archives of Microbiology · doi:10.1007/s002030050512 · Cited by 303
(2006) · ASM Press eBooks · doi:10.1128/9781555815714 · Cited by 260
Li, X., & Roseman, S. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0307645100 · Cited by 244
Natural chelating polymers : alginic acid, chitin, and chitosan
Müzzarelli, R. A. A. (1973) · Cited by 243
Santos, S. R., & Ochman, H. (2004) · Environmental Microbiology · doi:10.1111/j.1462-2920.2004.00617.x · Cited by 240
Two Chemically Distinct Pools of Organic Nitrogen Accumulate in the Ocean
Aluwihare, L. I., Repeta, D. J., Pantoja, S., & Johnson, C. G. (2005) · Science · doi:10.1126/science.1108925 · Cited by 207
Kita-Tsukamoto, K., Oyaizu, H., Nanba, K., & Simidu, U. (1993) · International Journal of Systematic Bacteriology · doi:10.1099/00207713-43-1-8 · Cited by 194
Bacteria of the γ-Subclass Proteobacteria Associated with Zooplankton in Chesapeake Bay
Heidelberg, J. F., Heidelberg, K. B., & Colwell, R. R. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.11.5498-5507.2002 · Cited by 189
Bassler, B. L., Yu, C., Lee, Y. C., & Roseman, S. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54225-3 · Cited by 170
Svitil, A. L., Chadhain, S. M. N., Moore, J. A., & Kirchman, D. L. (1997) · Applied and Environmental Microbiology · doi:10.1128/aem.63.2.408-413.1997 · Cited by 163
Adsorption and growth of Vibrio cholerae on chitin
Nalin, D. R., Daya, V., Reid, A. J., Levine, M. M., & Cisneros, L. (1979) · Infection and Immunity · doi:10.1128/iai.25.2.768-770.1979 · Cited by 157
Yang, C., Rodionov, D. A., Li, X., Laikova, O. N., Gelfand, M. S., Zagnitko, O. P., Romine, M. F., Obraztsova, A., Nealson, K. H., & Osterman, A. L. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m605052200 · Cited by 143
Riemann, L., & Azam, F. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.11.5554-5562.2002 · Cited by 136
Cottrell, M. T., Wood, D. N., Yu, L., & Kirchman, D. L. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.3.1195-1201.2000 · Cited by 134
New Aspects of the Extraction of Chitin from Squid Pens
Chaussard, G., & Domard, A. (2004) · Biomacromolecules · doi:10.1021/bm034401t · Cited by 132
The Chitin Catabolic Cascade in the Marine Bacterium Vibrio furnissii
Keyhani, N. O., & Roseman, S. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.52.33425 · Cited by 130
Reguera, G., & Kolter, R. (2005) · Journal of Bacteriology · doi:10.1128/jb.187.10.3551-3555.2005 · Cited by 128
The Chitin Catabolic Cascade in the Marine Bacterium Vibrio furnissii
Keyhani, N. O., & Roseman, S. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.52.33414 · Cited by 101
LeCleir, G. R., Buchan, A., & Hollibaugh, J. T. (2004) · Applied and Environmental Microbiology · doi:10.1128/aem.70.12.6977-6983.2004 · Cited by 98
Chitin Catabolism in the Marine Bacterium Vibrio furnissii
Keyhani, N. O., Li, X.-B., & Roseman, S. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m001041200 · Cited by 83
Hydrolysis and mineralization of chitin in the Delaware Estuary
Kirchman, D., & White, J. C. (1999) · Aquatic Microbial Ecology · doi:10.3354/ame018187 · Cited by 79
Dynamics ofVibrioPopulations and Their Role in Environmental Nutrient Cycling
Thompson, J. R., & Polz, M. F. (2014) · ASM Press eBooks · doi:10.1128/9781555815714.ch13 · Cited by 78
Use of a chiA probe for detection of chitinase genes in bacteria from the Chesapeake Bay1
Ramaiah, N., Hill, R. T., Chun, J., Ravel, J., Matté, M. H., Straube, W. L., & Colwell, R. R. (2000) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.2000.tb00755.x · Cited by 77
Effects of chitin and its soluble derivatives on survival of Vibrio cholerae O1 at low temperature
Amako, K., Shimodori, S., Imoto, T., Miake, S., & Umeda, A. (1987) · Applied and Environmental Microbiology · doi:10.1128/aem.53.3.603-605.1987 · Cited by 75
Chitin Catabolism in the Marine Bacterium Vibrio furnissii
Park, J. K., Keyhani, N. O., & Roseman, S. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m001042200 · Cited by 73
Orikoshi, H., Nakayama, S., Miyamoto, K., Hanato, C., Yasuda, M., Inamori, Y., & Tsujibo, H. (2005) · Applied and Environmental Microbiology · doi:10.1128/aem.71.4.1811-1815.2005 · Cited by 62
Sugar Transport by the Marine Chitinolytic Bacterium Vibrio furnissii
Bouma, C. L., & Roseman, S. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.52.33457 · Cited by 51
Lovley, D. R., Newman, D. K., Kennedy, E. K., Coates, J. D., Ahmann, D. M., Ellis, D. J., & Morel, F. M. M. (1997) · ScholarWorks@UMassAmherst (University of Massachusetts Amherst) · Cited by 46
Molecular Cloning and Characterization of a Unique β-Glucosidase from Vibrio cholerae
Park, J. K., Wang, L., Patel, H. V., & Roseman, S. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m202978200 · Cited by 31
Isolation of a Glucosamine-specific Kinase, a Unique Enzyme ofVibrio cholerae
Park, J. K., Wang, L., & Roseman, S. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m107953200 · Cited by 28
Flagged first, then by citations
Research works printed but not linked: 1
- 10.1016/S0022-2836(05)80360-2 · DOI · Paper page
Cited by
Sorted by citations
Bacterial chitin degradation—mechanisms and ecophysiological strategies
Beier, S., & Bertilsson, S. (2013) · Frontiers in Microbiology · doi:10.3389/fmicb.2013.00149 · Cited by 520
Resource Partitioning and Sympatric Differentiation Among Closely Related Bacterioplankton
Hunt, D. E., David, L. A., Gevers, D., Preheim, S. P., Alm, E. J., & Polz, M. F. (2008) · Science · doi:10.1126/science.1157890 · Cited by 501
Associations and dynamics of Vibrionaceae in the environment, from the genus to the population level
Takemura, A. F., Chien, D. M., & Polz, M. F. (2014) · Frontiers in Microbiology · doi:10.3389/fmicb.2014.00038 · Cited by 454
Chitin Deacetylases: Properties and Applications
Zhao, Y., Park, R., & Müzzarelli, R. A. A. (2010) · Marine Drugs · doi:10.3390/md8010024 · Cited by 348
Environmental reservoirs and mechanisms of persistence of Vibrio cholerae
Lutz, C., Erken, M., Noorian, P., Sun, S., & McDougald, D. (2013) · Frontiers in Microbiology · doi:10.3389/fmicb.2013.00375 · Cited by 289
Metadata
- Authors
- Hunt, D. E., Gevers, D., Vahora, N. M., & Polz, M. F.
- Affiliations
- Massachusetts Institute of Technology (US)
- Ghent University (BE)
- Corresponding author
- Polz, Martin F.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2008-01
- Volume
- 74
- Issue
- 1
- Pages
- 44-51
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC2223224
- OpenAlex
- W2096419043
- Cited by
- 236
- References
- 46
- Citations by year
- 2026: 14 · 2025: 8 · 2024: 12 · 2023: 23 · 2022: 12 · 2021: 15 · 2020: 14 · 2019: 11 · 2018: 19 · 2017: 10 · 2016: 14 · 2015: 19 · 2014: 9 · 2013: 12 · 2012: 8
- FWCI
- 3.18
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Flanders Marine Institute (Flanders Marine Institute) (publishedVersion, other-oa)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion, other-oa)
- Funders
- National Science Foundation
- U.S. Department of Energy (DEFG0208ER64516)
- Gordon and Betty Moore Foundation
- Vlaamse regering
- National Institute of Environmental Health Sciences
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Studies on Chitinases and Chitosanases
- Life Sciences › Immunology and Microbiology › Microbiology › Vibrio bacteria research studies
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Keywords
- chitin degradation, Vibrionaceae, chitinase A, chiA gene, Vibrio, Photobacterium, horizontal gene transfer, gene duplication, marine bacteria
- MeSH terms
- Acetylglucosamine, Bacterial Proteins, Chitin, Chitinases, DNA, Bacterial, Molecular Sequence Data, Phylogeny, Vibrionaceae, Conserved Sequence, Sequence Analysis, DNA, Metabolic Networks and Pathways
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
