The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Use of Ichip for High-Throughput In Situ Cultivation of “Uncultivable” Microbial Species
Open accessAbstract
One of the oldest unresolved microbiological phenomena is why only a small fraction of the diverse microbiological population grows on artificial media. The “uncultivable” microbial majority arguably represents our planet's largest unexplored pool of biological and chemical novelty. Previously we showed that species from this pool could be grown inside diffusion chambers incubated in situ , likely because diffusion provides microorganisms with their naturally occurring growth factors. Here we utilize this approach and develop a novel high-throughput platform for parallel cultivation and isolation of previously uncultivated microbial species from a variety of environments. We have designed and tested an isolation chip (ichip) composed of several hundred miniature diffusion chambers, each inoculated with a single environmental cell. We show that microbial recovery in the ichip exceeds manyfold that afforded by standard cultivation, and the grown species are of significant phylogenetic novelty. The new method allows access to a large and diverse array of previously inaccessible microorganisms and is well suited for both fundamental and applied research.
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Larsson, D. G. J., & Flach, C.-F. (2022) · Nature Reviews Microbiology · doi:10.1038/s41579-021-00649-x · Cited by 3,164
Antibiotics: past, present and future
Hutchings, M. I., Truman, A. W., & Wilkinson, B. (2019) · Current Opinion in Microbiology · doi:10.1016/j.mib.2019.10.008 · Cited by 2,370
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Lewis, K. (2013) · Nature Reviews Drug Discovery · doi:10.1038/nrd3975 · Cited by 1,445
Self-citationStewart, E. J. (2012) · Journal of Bacteriology · doi:10.1128/jb.00345-12 · Cited by 1,161
Core microbiomes for sustainable agroecosystems
Toju, H., Peay, K. G., Yamamichi, M., Narisawa, K., Hiruma, K., Naito, K., Fukuda, S., Ushio, M., Nakaoka, S., Onoda, Y., et al. (2018) · Nature Plants · doi:10.1038/s41477-018-0139-4 · Cited by 1,077
Metadata
- Authors
- Nichols, D., Cahoon, N., Trakhtenberg, E. M., Pham, L., Mehta, A., Belanger, A., Kanigan, T., Lewis, K., & Epstein, S. S.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2010-04-15
- Volume
- 76
- Issue
- 8
- Pages
- 2445-2450
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2129610424
- Cited by
- 732
- References
- 35
- FWCI
- 8.96
- Type
- journal article
- Language
- en
- Open access
- green
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- Nichols, D., Cahoon, N., Trakhtenberg, E. M., Pham, L., Mehta, A., Belanger, A., Kanigan, T., Lewis, K., & Epstein, S. S. (2010). Use of Ichip for High-Throughput In Situ Cultivation of “Uncultivable” Microbial Species. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.01754-09
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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