The DOI matches its Crossref record; 74 of the 74 references it cites were found in a registry or an index.
Growing Unculturable Bacteria
Open accessAbstract
The bacteria that can be grown in the laboratory are only a small fraction of the total diversity that exists in nature. At all levels of bacterial phylogeny, uncultured clades that do not grow on standard media are playing critical roles in cycling carbon, nitrogen, and other elements, synthesizing novel natural products, and impacting the surrounding organisms and environment. While molecular techniques, such as metagenomic sequencing, can provide some information independent of our ability to culture these organisms, it is essentially impossible to learn new gene and pathway functions from pure sequence data. A true understanding of the physiology of these bacteria and their roles in ecology, host health, and natural product production requires their cultivation in the laboratory. Recent advances in growing these species include coculture with other bacteria, recreating the environment in the laboratory, and combining these approaches with microcultivation technology to increase throughput and access rare species. These studies are unraveling the molecular mechanisms of unculturability and are identifying growth factors that promote the growth of previously unculturable organisms. This minireview summarizes the recent discoveries in this area and discusses the potential future of the field.
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Citations
Cites
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Cited by
Shotgun metagenomics, from sampling to analysis
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Culturing of ‘unculturable’ human microbiota reveals novel taxa and extensive sporulation
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Analysis of the microbiome: Advantages of whole genome shotgun versus 16S amplicon sequencing
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Culturing the human microbiota and culturomics
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Microbial siderophores and their potential applications: a review
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Metadata
- Authors
- Stewart, E. J.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2012-08-15
- Volume
- 194
- Issue
- 16
- Pages
- 4151-4160
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2152093511
- Cited by
- 1,161
- References
- 74
- FWCI
- 35.34
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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