Capillary Electrophoresis-Based Functional Genomics Screening to Discover Novel Archaeal DNA Modifying Enzymes

Asgard archaea illuminate the origin of eukaryotic cellular complexity

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Novel Division Level Bacterial Diversity in a Yellowstone Hot Spring

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Major New Microbial Groups Expand Diversity and Alter our Understanding of the Tree of Life

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The growing tree of Archaea: new perspectives on their diversity, evolution and ecology

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Quantifying the accessibility of the metagenome by random expression cloning techniques

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Intervening sequences in an Archaea DNA polymerase gene.

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Functional metagenomics of extreme environments

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Metagenomics of Thermophiles with a Focus on Discovery of Novel Thermozymes

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A novel DNA polymerase in the hyperthermophilic archaeon, Pyrococcus furiosus: gene cloning, expression, and characterization

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A Holliday junction resolvase from Pyrococcus furiosus : Functional similarity to Escherichia coli RuvC provides evidence for conserved mechanism of homologous recombination in Bacteria, Eukarya, and Archaea

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Structural and functional analysis of the archaeal endonuclease Nob1

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Identification of novel esterase-active enzymes from hot environments by use of the host bacterium Thermus thermophilus

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Expression of heterologous sigma factors enables functional screening of metagenomic and heterologous genomic libraries

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Identification of a mismatch-specific endonuclease in hyperthermophilic Archaea

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Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme

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Adapting capillary gel electrophoresis as a sensitive, high-throughput method to accelerate characterization of nucleic acid metabolic enzymes

Greenough, L., Schermerhorn, K. M., Mazzola, L., Bybee, J., Rivizzigno, D., Cantin, E., Slatko, B. E., & Gardner, A. F. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv899 · Cited by 65

Protein Synthesis Using a Reconstituted Cell‐Free System

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A DNA Ligase from a Hyperthermophilic Archaeon with Unique Cofactor Specificity

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A high-throughput assay for the comprehensive profiling of DNA ligase fidelity

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New Glycosidase Substrates for Droplet-Based Microfluidic Screening

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COMBREX-DB: an experiment centered database of protein function: knowledge, predictions and knowledge gaps

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Dark matter in archaeal genomes: a rich source of novel mobile elements, defense systems and secretory complexes

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Towards functional characterization of archaeal genomic dark matter

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Uracil-DNA glycosylase activities in hyperthermophilic micro-organisms

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Functional metagenomics identifies an exosialidase with an inverting catalytic mechanism that defines a new glycoside hydrolase family (GH156)

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Defining the RNaseH2 enzyme-initiated ribonucleotide excision repair pathway in Archaea

Heider, M. R., Burkhart, B. W., Santangelo, T. J., & Gardner, A. F. (2017) · Journal of Biological Chemistry · doi:10.1074/jbc.m117.783472 · Cited by 37

Biochemical reconstitution and genetic characterization of the major oxidative damage base excision DNA repair pathway in Thermococcus kodakarensis

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A Novel Single-Strand Specific 3′–5′ Exonuclease Found in the Hyperthermophilic Archaeon, Pyrococcus furiosus

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Chapter 44 Important but Little Known (or Forgotten) Artifacts in Protein Biochemistry

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Biochemical characterization and mutational studies of a thermostable uracil DNA glycosylase from the hyperthermophilic euryarchaeon Thermococcus barophilus Ch5

Shi, H., Gan, Q., Jiang, D., Wu, Y., Yin, Y., Hou, H., Chen, H., Xu, Y., Miao, L., Yang, Z., et al. (2019) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2019.05.073 · Cited by 9

Archaea as a Model System for Molecular Biology and Biotechnology

Lise, F. D., Iacono, R., Moracci, M., Strazzulli, A., & Cobucci‐Ponzano, B. (2023) · Biomolecules · doi:10.3390/biom13010114 · Cited by 34

High-throughput Kinetics using capillary Electrophoresis and Robotics (HiKER) platform used to study T7, T3, and Sp6 RNA polymerase misincorporation

Carter, Z. I., O’Brien, W. B., Lund, S. R., & Gardner, A. F. (2024) · PLoS ONE · doi:10.1371/journal.pone.0312743 · Cited by 4

High-throughput nanopore DNA sequencing of large insert fosmid clones directly from bacterial colonies

Chuzel, L., Sinha, A., Cunningham, C. V., & Taron, C. H. (2024) · Applied and Environmental Microbiology · doi:10.1128/aem.00243-24 · Cited by 3

Archaeal RNA processing and regulation: expanding the functional landscape

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Thermococcus kodakarensis TK0353 is a novel AP lyase with a new fold

Caffrey, P. J., Eckenroth, B. E., Burkhart, B. W., Zatopek, K. M., McClung, C. M., Santangelo, T. J., Doublié, S., & Gardner, A. F. (2023) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2023.105503 · Cited by 1

Ribonucleotide errors, RNase HII, and ribonucleotide excision repair

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A new method for the rapid identification of genes encoding restriction and modification enzymes. Nucleic Acids Res

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Functional screening of a soil metagenome for DNA endonucleases by acquired resistance to bacteriophage infection

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A simple, high sensitivity mutation screening using Ampligase mediated T7 endonuclease I and Surveyor nuclease with microfluidic capillary electrophoresis

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Adapting capillary gel electrophoresis as a sensitive, high-throughput method to accelerate characterization of nucleic acid metabolic enzymes

Greenough, L., Schermerhorn, K. M., Mazzola, L., Bybee, J., Rivizzigno, D., Cantin, E., Slatko, B. E., & Gardner, A. F. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv899 · Cited by 65

Functional Metagenomics: Construction and High-Throughput Screening of Fosmid Libraries for Discovery of Novel Carbohydrate-Active Enzymes

Ufarté, L., Bozonnet, S., Laville, É., Cecchini, D. A., Pizzut‐Serin, S., Jacquiod, S., Demanèche, S., Simonet, P., Franqueville, L., & Potocki-Véronèse, G. (2016) · Methods in molecular biology · doi:10.1007/978-1-4939-3369-3_15 · Cited by 10