The DOI matches its Crossref record.
Quantification of the Detrimental Effect of a Single Primer-Template Mismatch by Real-Time PCR Using the 16S rRNA Gene as an Example
Open accessAbstract
We investigated the effects of internal primer-template mismatches on the efficiency of PCR amplification using the 16S rRNA gene as the model template DNA. We observed that the presence of a single mismatch in the second half of the primer extension sequence can result in an underestimation of up to 1,000-fold of the gene copy number, depending on the primer and position of the mismatch.
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Cited by
Apprill, A., McNally, S., Parsons, R., & Weber, L. (2015) · Aquatic Microbial Ecology · doi:10.3354/ame01753 · Cited by 2,707
Stadhouders, R., Pas, S. D., Anber, J., Voermans, J., Mes, T. H. M., & Schutten, M. (2010) · The Journal of Molecular Diagnostics · doi:10.2353/jmoldx.2010.090035 · Cited by 399
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Evrony, G. D., Lee, E., Park, P. J., & Walsh, C. A. (2016) · eLife · doi:10.7554/elife.12966 · Cited by 163
Regulation of bacterial and fungal MCPA degradation at the soil–litter interface
Poll, C., Pagel, H., Devers-Lamrani, M., Martin-Laurent, F., Ingwersen, J., Streck, T., & Kandeler, E. (2010) · Soil Biology and Biochemistry · doi:10.1016/j.soilbio.2010.07.013 · Cited by 48
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Metadata
- Authors
- Bru, D., Martin-Laurent, F., & Philippot, L.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2008-03
- Volume
- 74
- Issue
- 5
- Pages
- 1660-1663
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2151308724
- Cited by
- 284
- References
- 0
- FWCI
- 4.84
- Type
- journal article
- Language
- en
- Open access
- green
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- Bru, D., Martin-Laurent, F., & Philippot, L. (2008). Quantification of the Detrimental Effect of a Single Primer-Template Mismatch by Real-Time PCR Using the 16S rRNA Gene as an Example. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.02403-07
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
