Cited by 28Microbial Metabolic Engineering and BioproductionBacterial Genetics and BiotechnologyMicrobial metabolism and enzyme function

Nitrogen Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing

Open access
Schmidt, Matthias, Pearson, Allison N., Incha, Matthew R., Thompson, Mitchell G., Baidoo, Edward E. K., Kakumanu, Ramu, Mukhopadhyay, Aindrila, Shih, Patrick M. +3 more·2022·Applied and Environmental Microbiology

Published version of a preprint

Abstract

Understanding the genetic basis of P. putida ’s diverse metabolism is imperative for us to reach its full potential as a host for metabolic engineering. Many target molecules of the bioeconomy and their precursors contain nitrogen.

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Crossref · 10.1128/aem.02430-21

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Authors
Schmidt, M., Pearson, A. N., Incha, M. R., Thompson, M. G., Baidoo, E. E. K., Kakumanu, R., Mukhopadhyay, A., Shih, P. M., Deutschbauer, A. M., Blank, L. M., & Keasling, J. D.
Affiliations
  • Lawrence Berkeley National Laboratory (US)
  • RWTH Aachen University (DE)
Corresponding author
Keasling, Jay D.
Venue
Applied and Environmental Microbiology
Published
2022-04-12
Volume
88
Issue
7
Pages
e0243021-e0243021
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W4221066590
Cited by
28
References
2
Citations by year
2026: 5 · 2025: 13 · 2024: 5 · 2023: 5
FWCI
1.88
Type
journal article
Language
en
Open access
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • eScholarship (California Digital Library) (submittedVersion, cc-by)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
  • RWTH Publications (RWTH Aachen) (submittedVersion)
Funders
  • Deutsche Forschungsgemeinschaft (FSC 2186)
  • U.S. Department of Energy (DE-AC02-05CH11231)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
Keywords
Pseudomonas putida, nitrogen metabolism, transposon mutagenesis, gene function annotation, transaminases, transcription factors, metabolic engineering
MeSH terms
Amino Acids, Transaminases, Nitrogen, Phenotype, Pseudomonas putida

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