The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli
Open accessAbstract
1-Butanol, an important chemical feedstock and advanced biofuel, is produced by Clostridium species. Various efforts have been made to transfer the clostridial 1-butanol pathway into other microorganisms. However, in contrast to similar compounds, only limited titers of 1-butanol were attained. In this work, we constructed a modified clostridial 1-butanol pathway in Escherichia coli to provide an irreversible reaction catalyzed by trans -enoyl-coenzyme A (CoA) reductase (Ter) and created NADH and acetyl-CoA driving forces to direct the flux. We achieved high-titer (30 g/liter) and high-yield (70 to 88% of the theoretical) production of 1-butanol anaerobically, comparable to or exceeding the levels demonstrated by native producers. Without the NADH and acetyl-CoA driving forces, the Ter reaction alone only achieved about 1/10 the level of production. The engineered host platform also enables the selection of essential enzymes with better catalytic efficiency or expression by anaerobic growth rescue. These results demonstrate the importance of driving forces in the efficient production of nonnative products.
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Cites
Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
Atsumi, S., Hanai, T., & Liao, J. C. (2008) · Nature · doi:10.1038/nature06450 · Cited by 1,945
Self-citationQureshi, N., Lolas, A., & Blaschek, H. P. (2001) · Journal of Industrial Microbiology and Biotechnology · doi:10.1038/sj.jim.7000131 · Cited by 147 · cited 1×
“coli achieved a level comparable to the levels produced by Clostridium species in flasks and batch fermentors (15, 18, 21, 34, 35), with productivity of 0.”
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Cited by
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Straathof, A. J. J. (2014) · Chemical Reviews · doi:10.1021/cr400309c · Cited by 459
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Liu, C.-G., Xue, C., Lin, Y.-H., & Bai, F.-W. (2013) · Biotechnology Advances · doi:10.1016/j.biotechadv.2012.11.005 · Cited by 307
Engineering microbes for tolerance to next-generation biofuels
Dunlop, M. J. (2011) · Biotechnology for Biofuels · doi:10.1186/1754-6834-4-32 · Cited by 293
ePathBrick: A Synthetic Biology Platform for Engineering Metabolic Pathways in E. coli
Xu, P., Vansiri, A., Bhan, N., & Koffas, M. A. G. (2012) · ACS Synthetic Biology · doi:10.1021/sb300016b · Cited by 255
Metadata
- Authors
- Shen, C. R., Lan, E. I., Dekishima, Y., Baez, A., Cho, K. M., & Liao, J. C.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2011-05
- Volume
- 77
- Issue
- 9
- Pages
- 2905-2915
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2160944601
- Cited by
- 623
- References
- 2
- FWCI
- 23.51
- Type
- journal article
- Language
- en
- Open access
- green
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- Shen, C. R., Lan, E. I., Dekishima, Y., Baez, A., Cho, K. M., & Liao, J. C. (2011). Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.03034-10
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