The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
High-Throughput Single-Cell Cultivation on Microfluidic Streak Plates
Open accessAbstract
This paper describes the microfluidic streak plate (MSP), a facile method for high-throughput microbial cell separation and cultivation in nanoliter sessile droplets. The MSP method builds upon the conventional streak plate technique by using microfluidic devices to generate nanoliter droplets that can be streaked manually or robotically onto petri dishes prefilled with carrier oil for cultivation of single cells. In addition, chemical gradients could be encoded in the droplet array for comprehensive dose-response analysis. The MSP method was validated by using single-cell isolation of Escherichia coli and antimicrobial susceptibility testing of Pseudomonas aeruginosa PAO1. The robustness of the MSP work flow was demonstrated by cultivating a soil community that degrades polycyclic aromatic hydrocarbons. Cultivation in droplets enabled detection of the richest species diversity with better coverage of rare species. Moreover, isolation and cultivation of bacterial strains by MSP led to the discovery of several species with high degradation efficiency, including four Mycobacterium isolates and a previously unknown fluoranthene-degrading Blastococcus species.
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Daniel, R. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1160 · Cited by 862
Zengler, K., Toledo, G., Rappé, M., Elkins, J., Mathur, E. J., Short, J. M., & Keller, M. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.252630999 · Cited by 796
The truth about metagenomics: quantifying and counteracting bias in 16S rRNA studies
Vaginal Microbiome Consortium (additional members), Brooks, J. P., Edwards, D. J., Harwich, M. D., Rivera, M. C., Fettweis, J. M., Serrano, M. G., Reris, R. A., Sheth, N. U., Huang, B., et al. (2015) · BMC Microbiology · doi:10.1186/s12866-015-0351-6 · Cited by 528
Cited by
Xu, X., Liu, W., Tian, S., Wang, W., Qi, Q., Jiang, P., Gao, X., Li, F., Li, H., & Yu, H. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.02885 · Cited by 688
Bacteria-driven phthalic acid ester biodegradation: Current status and emerging opportunities
Hu, R., Zhao, H., Xu, X., Wang, Z., Yu, K., Shu, L., Yan, Q., Wu, B., Mo, C., He, Z., et al. (2021) · Environment International · doi:10.1016/j.envint.2021.106560 · Cited by 176
Wang, C., Liu, H., Zhang, S., Ren, C., Xu, J., Chen, J., Chen, H., & Wu, W. (2025) · Nutrients · doi:10.3390/nu17020355 · Cited by 7
Metadata
- Authors
- Jiang, C.-Y., Dong, L., Zhao, J.-K., Hu, X., Shen, C., Qiao, Y., Zhang, X., Wang, Y., Ismagilov, R. F., Liu, S.-J., & Du, W.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2016-04
- Volume
- 82
- Issue
- 7
- Pages
- 2210-2218
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2285748537
- Cited by
- 184
- References
- 3
- FWCI
- 5.93
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Jiang, C.-Y., Dong, L., Zhao, J.-K., Hu, X., Shen, C., Qiao, Y., Zhang, X., Wang, Y., Ismagilov, R. F., Liu, S.-J., & Du, W. (2016). High-Throughput Single-Cell Cultivation on Microfluidic Streak Plates. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.03588-15
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