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Cited by 356

The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.

Fermentation of cellulose and cellobiose by Clostridium thermocellum in the absence of Methanobacterium thermoautotrophicum

Open access
Weimer, P J, Zeikus, J G·1977·Applied and Environmental Microbiology

Abstract

The fermentation of cellulose and cellobiose by Clostridium thermocellum monocultures and C. thermocellum/Methanobacterium thermoautotrophicum cocultures was studied. All cultures were grown under anaerobic conditions in batch culture at 60 degrees C. When grown on cellulose, the coculture exhibited a shorter lag before initiation and growth and celluloysis than did the monoculture. Cellulase activity appeared earlier in the coculture than in the monoculture; however, after growth had ceased, cellulase activity was greater in the monoculture. Monocultures produced primarily ethanol, acetic acid, H2 and CO2. Cocultures produced more H2 and acetic acid and less ethanol than did the monoculture. In the coculture, conversion of H2 to methane was usually complete, and most of the methane produced was derived from CO2 reduction rather than from acetate conversion. Agents of fermentation stoppage were found to be low pH and high concentrations of ethanol in the monoculture and low pH in the coculture. Fermentation of cellobiose was more rapid than that of cellulose. In cellobiose medium, the methanogen caused only slight changes in the fermentation balance of the Clostridium, and free H2 was produced.

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Crossref · 10.1128/aem.33.2.289-297.1977

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Metadata

Authors
Weimer, P. J., & Zeikus, J. G.
Venue
Applied and Environmental Microbiology
Published
1977-02
Volume
33
Issue
2
Pages
289-297
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W1506525126
Cited by
356
References
21
FWCI
15.06
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Fermentation of cellulose and cellobiose by Clostridium thermocellum in the absence of Methanobacterium thermoautotrophicum. https://app.cochat.ai/papers/10.1128/aem.33.2.289-297.1977
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Weimer, P. J., & Zeikus, J. G. (1977). Fermentation of cellulose and cellobiose by Clostridium thermocellum in the absence of Methanobacterium thermoautotrophicum. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.33.2.289-297.1977

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