Use of nuclepore filters for counting bacteria by fluorescence microscopy
Open accessAbstract
Polycarbonate Nuclepore filters are better than cellulose filters for the direct counting of bacteria because they have uniform pore size and a flat surface that retains all of the bacteria on top of the filter. Although cellulose filters also retain all of the bacteria, many are trapped inside the filter where they cannot be counted. Before use, the Nuclepore filters must be dyed with irgalan black to eliminate autofluorescence. Direct counts of bacteria in lake and ocean waters are twice as high with Nuclepore filters as with cellulose filters.
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- Authors
- Hobbie, J. E., Daley, R. J., & Jasper, S.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1977-05
- Volume
- 33
- Issue
- 5
- Pages
- 1225-1228
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC170856
- OpenAlex
- W1515985097
- Cited by
- 4,244
- References
- 0
- Citations by year
- 2026: 13 · 2025: 14 · 2024: 29 · 2023: 21 · 2022: 32 · 2021: 42 · 2020: 40 · 2019: 52 · 2018: 71 · 2017: 60 · 2016: 55 · 2015: 65 · 2014: 74 · 2013: 67 · 2012: 74
- FWCI
- 38.52
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Physical Sciences › Environmental Science › Water Science and Technology › Fecal contamination and water quality
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal ecosystems
- Keywords
- Nuclepore filters, polycarbonate filters, cellulose filters, bacterial enumeration, fluorescence microscopy, autofluorescence, filter pore size, lake water, ocean water
- MeSH terms
- Bacteria, Bacteriological Techniques, Evaluation Studies as Topic, Fresh Water, Micropore Filters, Microscopy, Fluorescence, Seawater, Water Microbiology
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