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Saccharification of Complex Cellulosic Substrates by the Cellulase System from Clostridium thermocellum
Open accessAbstract
True cellulase activity has been demonstrated in cell-free preparations from the thermophilic anaerobe Clostridium thermocellum . Such activity depends upon the presence of Ca 2+ and a thiol-reducing agent of which dithiothreitol is the most promising. Under these conditions, native (cotton) and derived forms of cellulose (Avicel and filter paper) were all extensively solubilized at rates comparable with cellulase from Trichoderma reesei . Maximum activity of the Clostridium cellulase was displayed at 70°C and at pH 5.7 and 6.1 on Avicel and carboxymethylcellulose, respectively. In the absence of substrate at temperatures up to 70°C, carboxymethylcellulase was much more unstable than the Avicel-hydrolyzing activity.
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Citations
Cited by
Microbial Cellulose Utilization: Fundamentals and Biotechnology
Lynd, L. R., Weimer, P. J., van Zyl, W. H., & Pretorius, I. S. (2002) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.66.3.506-577.2002 · Cited by 4,726
Lynd, L. R., Wyman, C. E., & Gerngross, T. U. (1999) · Biotechnology Progress · doi:10.1021/bp990109e · Cited by 718
Adherence of Clostridium thermocellum to cellulose
Bayer, E. A., Kenig, R., & Lamed, R. (1983) · Journal of Bacteriology · doi:10.1128/jb.156.2.818-827.1983 · Cited by 318
Metadata
- Authors
- Johnson, E. A., Sakajoh, M., Halliwell, G., Madia, A., & Demain, A. L.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1982-05
- Volume
- 43
- Issue
- 5
- Pages
- 1125-1132
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1593379289
- Cited by
- 326
- References
- 0
- FWCI
- 17.10
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Saccharification of Complex Cellulosic Substrates by the Cellulase System from Clostridium thermocellum. https://app.cochat.ai/papers/10.1128/aem.43.5.1125-1132.1982
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- Johnson, E. A., Sakajoh, M., Halliwell, G., Madia, A., & Demain, A. L. (1982). Saccharification of Complex Cellulosic Substrates by the Cellulase System from Clostridium thermocellum. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.43.5.1125-1132.1982
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