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Construction of plasmid cloning vectors for lactic streptococci which also replicate in Bacillus subtilis and Escherichia coli
Open accessAbstract
The cryptic Streptococcus cremoris Wg2 plasmid pWV01 (1.5 megadaltons) was genetically marked with the chloramphenicol resistance (Cmr) gene from pC194. The recombinant plasmid (pGK1, 2.4 megadaltons) replicated and expressed Cmr in Bacillus subtilis. From this plasmid an insertion-inactivation vector was constructed by inserting the erythromycin resistance (Emr) gene from pE194 cop-6. This plasmid (pGK12, 2.9 megadaltons) contained a unique BclI site in the Emr gene and unique ClaI and HpaII sites outside both resistance genes. It was stably maintained in B. subtilis at a copy number of approximately 5. pGK12 also transformed Escherichia coli competent cells to Cmr and Emr. The copy number in E. coli was about 60. Moreover, pGK12 transformed protoplasts of Streptococcus lactis. In this host both resistance genes are expressed. pGK12 is stably maintained in S. lactis at a copy number of 3.
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Cited by
Efficient insertional mutagenesis in lactococci and other gram-positive bacteria
Maguin, E., Prévost, H., Ehrlich, S. D., & Gruss, A. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.3.931-935.1996 · Cited by 486
The Genera Lactobacillus and Carnobacterium
(2011) · SpringerReference · doi:10.1007/springerreference_3731 · Cited by 382
Proteolytic enzymes of lactic acid bacteria
Law, J., & Haandrikman, A. (1997) · International Dairy Journal · doi:10.1016/0958-6946(95)00073-9 · Cited by 225
The proteolytic system of lactic acid bacteria
Kok, J., & De Vos, W. M. (1994) · Genetics and Biotechnology of Lactic Acid Bacteria · doi:10.1007/978-94-011-1340-3_4 · Cited by 205
Self-citationCogan, T. M., & Hill, C. (1993) · Cheese: Chemistry, Physics and Microbiology · doi:10.1007/978-1-4615-2650-6_6 · Cited by 139
Metadata
- Authors
- Kok, J., van der Vossen, J. M., & Venema, G.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1984-10
- Volume
- 48
- Issue
- 4
- Pages
- 726-731
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2156524114
- Cited by
- 381
- References
- 0
- FWCI
- 4.81
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Kok, J., van der Vossen, J. M., & Venema, G. (1984). Construction of plasmid cloning vectors for lactic streptococci which also replicate in Bacillus subtilis and Escherichia coli. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.48.4.726-731.1984
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