The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Microbial metabolism of polycyclic aromatic hydrocarbons: isolation and characterization of a pyrene-degrading bacterium
Open accessAbstract
Microbiological analyses of sediments located near a point source for petrogenic chemicals resulted in the isolation of a pyrene-mineralizing bacterium. This isolate was identified as a Mycobacterium sp. on the basis of its cellular and colony morphology, gram-positive and strong acid-fast reactions, diagnostic biochemical tests, 66.6% G + C content of the DNA, and high-molecular-weight mycolic acids (C58 to C64). The mycobacterium mineralized pyrene when grown in a mineral salts medium supplemented with nutrients but was unable to utilize pyrene as a sole source of carbon and energy. The mycobacterium grew well at 24 and 30 degrees C and minimally at 35 degrees C. No growth was observed at 5 or 42 degrees C. The mycobacterium grew well at salt concentrations up to 4%. Pyrene-induced Mycobacterium cultures mineralized 5% of the pyrene after 6 h and reached a maximum of 48% mineralization within 72 h. Treatment of induced and noninduced cultures with chloramphenicol showed that pyrene-degrading enzymes were inducible in this Mycobacterium sp. This bacterium could also mineralize other polycyclic aromatic hydrocarbons and alkyl- and nitro-substituted polycyclic aromatic hydrocarbons including naphthalene, phenanthrene, fluoranthene, 3-methylcholanthrene, 1-nitropyrene, and 6-nitrochrysene. This is the first report of a bacterium able to extensively mineralize pyrene and other polycyclic aromatic hydrocarbons containing four aromatic rings.
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Cites
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Self-citationMINNIKIN, D. E., ALSHAMAONY, L., & GOODFELLOW, M. (1975) · Journal of General Microbiology · doi:10.1099/00221287-88-1-200 · Cited by 514
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Cited by
Epidemiology of infection by nontuberculous mycobacteria
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Self-citationBacterial Degradation of Aromatic Compounds
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Microbial biodegradation of polyaromatic hydrocarbons
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Biodegradation of halogenated organic compounds
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Metadata
- Authors
- Heitkamp, M. A., Franklin, W., & Cerniglia, C. E.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1988-10
- Volume
- 54
- Issue
- 10
- Pages
- 2549-2555
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1494340495
- Cited by
- 302
- References
- 20
- FWCI
- 8.25
- Type
- journal article
- Language
- en
- Open access
- green
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- Heitkamp, M. A., Franklin, W., & Cerniglia, C. E. (1988). Microbial metabolism of polycyclic aromatic hydrocarbons: isolation and characterization of a pyrene-degrading bacterium. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.54.10.2549-2555.1988
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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