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Fundamental Procedures for Determining Ergosterol Content of Decaying Plant Material by Liquid Chromatography
Open accessAbstract
Portions of published procedures for measurement of ergosterol content of decomposing plants were examined for their influence upon ergosterol yield. Common methods of treatment of plant samples prior to sterol extraction (e.g., oven drying, freezing, lyophilization) led to reduced recoveries of ergosterol (ca. 20 to 80%). The least destructive method was direct placement and storage in methanol. Photoconversion of ergosterol is not likely to cause losses during analysis, but losses are likely if there is insufficient mixing during neutral-lipid partitioning from base-hydrolysis reagents. Homogenization (two times for 2 min) and refluxing (2 h) in methanol were equally effective in extracting ergosterol. Direct extraction in base-hydrolysis reagents was less effective (by ca. 40%).
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Cited by
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Nutrient limitation of epilithic and epixylic biofilms in ten North American streams
Tank, J. L., & Dodds, W. K. (2003) · Freshwater Biology · doi:10.1046/j.1365-2427.2003.01067.x · Cited by 360
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Weete, J. D., Abril, M., & Blackwell, M. (2010) · PLoS ONE · doi:10.1371/journal.pone.0010899 · Cited by 316
Microbial characteristics of soil quality
Kennedy, A. C., & Papendick, R. I. (1995) · Journal of Soil and Water Conservation · doi:10.1080/00224561.1995.12456957 · Cited by 274
5 Ergosterol Analysis as a Means of Quantifying Mycorrhizal Biomass
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Metadata
- Authors
- Newell, S. Y., Arsuffi, T. L., & Fallon, R. D.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1988-07
- Volume
- 54
- Issue
- 7
- Pages
- 1876-1879
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2154640684
- Cited by
- 263
- References
- 0
- FWCI
- 1.52
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Newell, S. Y., Arsuffi, T. L., & Fallon, R. D. (1988). Fundamental Procedures for Determining Ergosterol Content of Decaying Plant Material by Liquid Chromatography. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.54.7.1876-1879.1988
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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