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Detection and activity of a bacteriocin produced by Leuconostoc mesenteroides
Open accessAbstract
Leuconostoc mesenteroides UL5 was found to produce a bacteriocin, referred as mesenterocin 5, active against Listeria monocytogenes strains but with no effect on several useful lactic acid bacteria. The antimicrobial substance is a protein, since its activity was completely destroyed following protease (pronase) treatment. However, it was relatively heat stable (100 degrees C for 30 min) and partially denaturated by chloroform. The inhibitory effect of the bacteriocin on sensitive bacterial strains was determined by a critical-dilution micromethod. Mutants of L. mesenteroides UL5 which had lost the capacity to produce the bacteriocin were obtained. The mutant strain was stable and phenotypically identical to parental cells and remained resistant to the bacteriocin. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to detect bacteriocin activity corresponding to an apparent molecular mass of about 4.5 kDa.
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Citations
Cited by
Biopreservation by lactic acid bacteria
Stiles, M. E. (1996) · Antonie van Leeuwenhoek · doi:10.1007/bf00395940 · Cited by 733
Bacteriocins from lactic acid bacteria: purification, properties and use as biopreservatives
Parada, J. L., Caron, C. R., Medeiros, A. B. P., & Soccol, C. R. (2007) · Brazilian Archives of Biology and Technology · doi:10.1590/s1516-89132007000300018 · Cited by 357
Dellaglio, F., Dicks, L. M. T., & Torriani, S. (1995) · The Genera of Lactic Acid Bacteria · doi:10.1007/978-1-4615-5817-0_7 · Cited by 108
(2006) · Microbial Cell Factories · doi:10.1186/1475-2859-5-30 · Cited by 50
Metadata
- Authors
- Daba, H., Pandian, S., Gosselin, J. F., Simard, R. E., Huang, J., & Lacroix, C.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1991-12
- Volume
- 57
- Issue
- 12
- Pages
- 3450-3455
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1540726219
- Cited by
- 211
- References
- 0
- FWCI
- 20.67
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Daba, H., Pandian, S., Gosselin, J. F., Simard, R. E., Huang, J., & Lacroix, C. (1991). Detection and activity of a bacteriocin produced by Leuconostoc mesenteroides. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.57.12.3450-3455.1991
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