Growth of and omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions
Open accessAbstract
Detailed studies were carried out on the effects of nitrogen source, phosphate, sodium chloride, growth factors, precursors, CO2, temperature, initial pH, and inoculum size on biomass and eicosapentaenoic acid (EPA) production by Phaeodactylum tricornutum. The EPA content of total fatty acids increased with increasing concentrations of nitrate and urea. Sodium chloride was not required for growth or EPA production. While vitamins B1 and B12 did not affect growth significantly, EPA yield was increased by 65% by B12 supplementation. Maximum EPA production occurred when the air gassing supply was supplemented with 1% CO2. Optimum culture temperature and initial pH for EPA production were 21.5 to 23 degrees C and 7.6, respectively. EPA yields of up to 133 mg/liter of culture were observed. EPA constituted up to 30 to 40% of total fatty acids.
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Algae growth and production of Omega-3 polyunsaturated fatty acids is greatly influenced by carbon sources and light strength.
“The algae growth and productions of Omega-3 Polyunsaturated fatty acids is greatly influence by carbon sources and light strength [201–203].”
From The promising future of microalgae: Current status, challenges, and optimization of a sustainable and renewable industry for biofuels, feed, and other products (2018) · cites this paper as [202]
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References 34 · Cited by 371
- Authors
- Yongmanitchai, W., & Ward, O. P.
- Affiliations
- University of Waterloo (CA)
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1991-02
- Volume
- 57
- Issue
- 2
- Pages
- 419-425
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC182726
- OpenAlex
- W1602683653
- Cited by
- 371
- References
- 34
- Citations by year
- 2026: 5 · 2025: 9 · 2024: 9 · 2023: 5 · 2022: 14 · 2021: 15 · 2020: 28 · 2019: 23 · 2018: 19 · 2017: 29 · 2016: 15 · 2015: 22 · 2014: 19 · 2013: 22 · 2012: 20
- FWCI
- 8.46
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Phaeodactylum tricornutum, eicosapentaenoic acid, omega-3 fatty acids, microalgae cultivation, nitrogen source, nitrate, urea, phosphate, sodium chloride, vitamin B12, thiamine, carbon dioxide supplementation, culture temperature, initial pH, inoculum size, biomass
- MeSH terms
- Culture Media, Hydrogen-Ion Concentration, Temperature, Eicosapentaenoic Acid, Fatty Acids, Omega-3, Eukaryota
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