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Detection of Escherichia coli and Shigella spp. in water by using the polymerase chain reaction and gene probes for uid

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Bej, A K, DiCesare, J L, Haff, L, Atlas, R M·1991·Applied and Environmental Microbiology

Abstract

A method was developed for the detection of the fecal coliform bacterium Escherichia coli, using the polymerase chain reaction and gene probes, based on amplifying regions of the uid gene that code for beta-glucuronidase, expression of which forms the basis for fecal coliform detection by the commercially available Colilert method. Amplification and gene probe detection of four different regions of uid specifically detected E. coli and Shigella species, including beta-glucuronidase-negative strains of E. coli; no amplification was observed for other coliform and nonenteric bacteria.

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Crossref · 10.1128/aem.57.4.1013-1017.1991

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Metadata

Authors
Bej, A. K., DiCesare, J. L., Haff, L., & Atlas, R. M.
Venue
Applied and Environmental Microbiology
Published
1991-04
Volume
57
Issue
4
Pages
1013-1017
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W2121731497
Cited by
347
References
0
FWCI
30.52
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Detection of Escherichia coli and Shigella spp. in water by using the polymerase chain reaction and gene probes for uid. https://app.cochat.ai/papers/10.1128/aem.57.4.1013-1017.1991
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Bej, A. K., DiCesare, J. L., Haff, L., & Atlas, R. M. (1991). Detection of Escherichia coli and Shigella spp. in water by using the polymerase chain reaction and gene probes for uid. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.57.4.1013-1017.1991

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