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Cited by 43Antibiotic Resistance in BacteriaPharmaceutical and Antibiotic Environmental ImpactsSalmonella and Campylobacter epidemiology

Multidrug-resistant extended spectrum β-lactamase (ESBL)-producing Escherichia coli from farm produce and agricultural environments in Edo State, Nigeria

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Igbinosa, Etinosa O., Beshiru, Abeni, Igbinosa, Isoken H., Cho, Gyu-Sung, Franz, Charles M. A. P.·2023·PLOS ONE
DOI 10.1371/journal.pone.0282835PMC PMC10004523
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Abstract

Antimicrobial resistance (AMR) is a major public health concern, especially the extended-spectrum β-lactamase-producing (ESBL) Escherichia coli bacteria are emerging as a global human health hazard. This study characterized extended-spectrum β-lactamase Escherichia coli (ESBL-E. coli) isolates from farm sources and open markets in Edo State, Nigeria. A total of 254 samples were obtained in Edo State and included representatives from agricultural farms (soil, manure, irrigation water) and vegetables from open markets, which included ready-to-eat (RTE) salads and vegetables which could potentially be consumed uncooked. Samples were culturally tested for the ESBL phenotype using ESBL selective media, and isolates were further identified and characterized via polymerase chain reaction (PCR) for β-lactamase and other antibiotic resistance determinants. ESBL E. coli strains isolated from agricultural farms included 68% (17/25) from the soil, 84% (21/25) from manure and 28% (7/25) from irrigation water and 24.4% (19/78) from vegetables. ESBL E. coli were also isolated from RTE salads at 20% (12/60) and vegetables obtained from vendors and open markets at 36.6% (15/41). A total of 64 E. coli isolates were identified using PCR. Upon further characterization, 85.9% (55/64) of the isolates were resistant to ≥ 3 and ≤ 7 antimicrobial classes, which allows for characterizing these as being multidrug-resistant. The MDR isolates from this study harboured ≥1 and ≤5 AMR determinants. The MDR isolates also harboured ≥1 and ≤3 beta-lactamase genes. Findings from this study showed that fresh vegetables and salads could be contaminated with ESBL-E. coli, particularly fresh produce from farms that use untreated water for irrigation. Appropriate measures, including improving irrigation water quality and agricultural practices, need to be implemented, and global regulatory guiding principles are crucial to ensure public health and consumer safety.

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  • Studies have revealed the presence of MDR bacteria, including methicillin-resistant S. aureus (MRSA), ESBL-producing Enterobacteriaceae, and MDR-Salmonella, in meat products.

    “Studies have revealed the presence of MDR bacteria, including methicillin-resistant S. aureus (MRSA), ESBL-producing Enterobacteriaceae, and MDR-Salmonella, in meat products [63,76,77,78,79].”

    From A Systematic Review on Antimicrobial Resistance in Ghana from a One Health Perspective (2024) · cites this paper as [78]

    The Discussion (page not provided) reports multidrug-resistant ESBL-producing E. coli in fresh produce, supporting the claim's general point that food can carry MDR bacteria. It does not establish their presence in meat products or report MRSA or MDR Salmonella in meat. (LLM, conf=0.86)

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Authors
Igbinosa, E. O., Beshiru, A., Igbinosa, I. H., Cho, G.-S., & Franz, C. M. A. P.
Affiliations
  • University of Benin (NG)
  • Western Delta University (NG)
  • Max Rubner Institut (DE)
Corresponding author
  • Igbinosa, Etinosa O.
  • Franz, Charles M. A. P.
ORCID
Venue
PLOS ONE
Publisher
Public Library of Science (PLoS)
Published
2023-03-10
Volume
18
Issue
3
Pages
e0282835
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0282835
PMC
PMC10004523
OpenAlex
W4323805620
Cited by
43
References
1
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Type
journal article
Language
en
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  • PLoS ONE (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Alexander von Humboldt-Stiftung (NGA / 1163897)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
  • Physical Sciences › Environmental Science › Pollution › Pharmaceutical and Antibiotic Environmental Impacts
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Salmonella and Campylobacter epidemiology
Keywords
extended-spectrum beta-lactamase, Escherichia coli, multidrug resistance, antimicrobial resistance, foodborne pathogens, fresh produce contamination, ready-to-eat salads, vegetables, soil, manure, irrigation water, antibiotic resistance genes, beta-lactamase genes, Edo State
MeSH terms
Farms, Anti-Bacterial Agents, beta-Lactamases, Escherichia coli, Escherichia coli Infections, Humans, Manure, Nigeria, Soil

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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