The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Adsorption of plasmid DNA to mineral surfaces and protection against DNase I
Open accessAbstract
The adsorption of [3H]thymidine-labeled plasmid DNA (pHC314; 2.4 kb) of different conformations to chemically pure sand was studied in a flowthrough microenvironment. The extent of adsorption was affected by the concentration and valency of cations, indicating a charge-dependent process. Bivalent cations (Mg2+, Ca2+) were 100-fold more effective than monovalent cations (Na+, K+, NH4+). Quantitative adsorption of up to 1 microgram of negatively supercoiled or linearized plasmid DNA to 0.7 g of sand was observed in the presence of 5 mM MgCl2 at pH 7. Under these conditions, more than 85% of DNA adsorbed within 60 s. Maximum adsorption was 4 micrograms of DNA to 0.7 g of sand. Supercoil molecules adsorbed slightly less than linearized or open circular plasmids. An increase of the pH from 5 to 9 decreased adsorption at 0.5 mM MgCl2 about eightfold. It is concluded that adsorption of plasmid DNA to sand depends on the neutralization of negative charges on the DNA molecules and the mineral surfaces by cations. The results are discussed on the grounds of the polyelectrolyte adsorption model. Sand-adsorbed DNA was 100 times more resistant against DNase I than was DNA free in solution. The data support the idea that plasmid DNA can enter the extracellular bacterial gene pool which is located at mineral surfaces in natural bacterial habitats.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Studies on transformation of Escherichia coli with plasmids
Hanahan, D. (1983) · Journal of Molecular Biology · doi:10.1016/s0022-2836(83)80284-8 · Cited by 11,157
A simple, rapid, and sensitive DNA assay procedure
Labarca, C., & Paigen, K. (1980) · Analytical Biochemistry · doi:10.1016/0003-2697(80)90165-7 · Cited by 4,901
Adsorption of DNA to sand and variable degradation rates of adsorbed DNA
Lorenz, M. G., & Wackernagel, W. (1987) · Applied and Environmental Microbiology · doi:10.1128/aem.53.12.2948-2952.1987 · Cited by 363
Self-citationStotzky, G. (1986) · SSSA Special Publications · doi:10.2136/sssaspecpub17.c10 · Cited by 318
Bruce Wallace, R., Schold, M., Johnson, M. J., Dembek, P., & Itakura, K. (1981) · Nucleic Acids Research · doi:10.1093/nar/9.15.3647 · Cited by 202
Protection of Sediment-Adsorbed Transforming DNA Against Enzymatic Inactivation
Aardema, B. W., Lorenz, M. G., & Krumbein, W. E. (1983) · Applied and Environmental Microbiology · doi:10.1128/aem.46.2.417-420.1983 · Cited by 128
Self-citationHighly Efficient Genetic Transformation of Bacillus subtilis Attached to Sand Grains
Lorenz, M. G., Aardema, B. W., & Wackernagel, W. (1988) · Microbiology · doi:10.1099/00221287-134-1-107 · Cited by 126
Self-citationNatural genetic transformation of Pseudomonas stutzeri by sand-adsorbed DNA
Lorenz, M. G., & Wackernagel, W. (1990) · Archives of Microbiology · doi:10.1007/bf00276535 · Cited by 103
Self-citation
Cited by
Environmental Conditions Influence eDNA Persistence in Aquatic Systems
Barnes, M. A., Turner, C. R., Jerde, C. L., Renshaw, M. A., Chadderton, W. L., & Lodge, D. M. (2014) · Environmental Science & Technology · doi:10.1021/es404734p · Cited by 939
Nnadozie, C. F., & Odume, O. N. (2019) · Environmental Pollution · doi:10.1016/j.envpol.2019.113067 · Cited by 365
TABERLET, P., PRUD’HOMME, S. M., CAMPIONE, E., ROY, J., MIQUEL, C., SHEHZAD, W., GIELLY, L., RIOUX, D., CHOLER, P., CLÉMENT, J., et al. (2012) · Molecular Ecology · doi:10.1111/j.1365-294x.2011.05317.x · Cited by 353
Water−Silica Force Field for Simulating Nanodevices
Cruz-Chu, E. R., Aksimentiev, A., & Schulten, K. (2006) · The Journal of Physical Chemistry B · doi:10.1021/jp063896o · Cited by 323
Cyanobacterial Mats and Stromatolites
Stal, L. J. (2012) · Ecology of Cyanobacteria II · doi:10.1007/978-94-007-3855-3_4 · Cited by 305
Metadata
- Authors
- Romanowski, G., Lorenz, M. G., & Wackernagel, W.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1991-04
- Volume
- 57
- Issue
- 4
- Pages
- 1057-1061
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1587890895
- Cited by
- 332
- References
- 10
- FWCI
- 8.06
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Adsorption of plasmid DNA to mineral surfaces and protection against DNase I. https://app.cochat.ai/papers/10.1128/aem.57.4.1057-1061.1991
- The paper
- Romanowski, G., Lorenz, M. G., & Wackernagel, W. (1991). Adsorption of plasmid DNA to mineral surfaces and protection against DNase I. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.57.4.1057-1061.1991
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
