The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification
Open accessAbstract
Systematic computer alignment of mycoplasmal 16S rRNA sequences allowed the identification of variable regions with both genus- and species-specific sequences. Species-specific sequences of Mycoplasma collis were elucidated by asymmetric amplification and dideoxynucleotide sequencing of variable regions, using primers complementary to conserved regions of 16S rRNA. Primers selected for Mycoplasma pneumoniae, M. hominis, M. fermentans, Ureaplasma urealyticum, M. pulmonis, M. arthritidis, M. neurolyticum, M. muris, and M. collis proved to be species specific in the polymerase chain reaction. The genus-specific primers reacted with all mycoplasmal species investigated as well as with members of the genera Ureaplasma, Spiroplasma, and Acholeplasma. No cross-reaction was observed with members of the closely related genera Streptococcus, Lactobacillus, Bacillus, and Clostridium or with any other microorganism tested. On the basis of the high copy number of rRNA, a highly sensitive polymerase chain reaction assay was developed in which the nucleic acid content equivalent to a single organism could be detected.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
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Detection of Mycoplasma in cell cultures
Young, L., Sung, J., Stacey, G., & Masters, J. R. (2010) · Nature Protocols · doi:10.1038/nprot.2010.43 · Cited by 437
Laboratory diagnosis of Mycoplasma pneumoniae infection
Daxboeck, F., Krause, R., & Wenisch, C. (2003) · Clinical Microbiology and Infection · doi:10.1046/j.1469-0691.2003.00590.x · Cited by 349
Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR
van Kuppeveld, F. J., Johansson, K. E., Galama, J. M., Kissing, J., Bölske, G., van der Logt, J. T., & Melchers, W. J. (1994) · Applied and Environmental Microbiology · doi:10.1128/aem.60.1.149-152.1994 · Cited by 186
Self-citationPollack, J. D., Williams, M. V., & McElhaney, R. N. (1997) · Critical Reviews in Microbiology · doi:10.3109/10408419709115140 · Cited by 180
Martins-Neves, S. R., Paiva-Oliveira, D. I., Fontes-Ribeiro, C., Bovée, J. V. M. G., Cleton-Jansen, A.-M., & Gomes, C. M. F. (2018) · Cancer Letters · doi:10.1016/j.canlet.2017.11.004 · Cited by 115
Metadata
- Authors
- van Kuppeveld, F. J., van der Logt, J. T., Angulo, A. F., van Zoest, M. J., Quint, W. G., Niesters, H. G., Galama, J. M., & Melchers, W. J.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1992-08
- Volume
- 58
- Issue
- 8
- Pages
- 2606-2615
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2120316065
- Cited by
- 503
- References
- 1
- FWCI
- 11.10
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- van Kuppeveld, F. J., van der Logt, J. T., Angulo, A. F., van Zoest, M. J., Quint, W. G., Niesters, H. G., Galama, J. M., & Melchers, W. J. (1992). Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.58.8.2606-2615.1992
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