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Influence of Ammonium Salts and Cane Molasses on Growth of Alcaligenes eutrophus and Production of Polyhydroxybutyrate
Open accessAbstract
The production of polyhydroxybutyrate (PHB) by Alcaligenes eutrophus DSM 545 was studied in a synthetic medium with 3% glucose at pH 7.0 supplemented with several ammonium substrates and cane molasses. Growth was measured by dry cell weight, and the PHB content was measured by gas chromatography. The effects of ammonium sources such as sulfate, nitrate, phosphate, and chloride salts and those of different ammonium sulfate concentrations were evaluated. The best growth and PHB production were obtained with ammonium sulfate; however, NH(inf4)(sup+) concentrations between 0.5 and 1.5 g/liter showed no significant difference. Ammonium sulfate was therefore used as the sole source of NH(inf4)(sup+) for experiments with cane molasses as the growth activator. Optimal growth and PHB production were obtained with 0.3% molasses. However, the yields of biomass (39 to 48%) and PHB (17 to 26%) varied significantly among the different ammonium substrates and cane molasses concentrations.
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“Moreover, molasses and corn steep liquor are prominent culture media in fermentative processes due to the high content of sugars and nitrogen, respectively (Beaulieu et al., 1995).”
From Response surface optimization of D(-)-lactic acid production by Lactobacillus SMI8 using corn steep liquor and yeast autolysate as an alternative nitrogen source (2009) · cites this paper as Beaulieu et al., 1995
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Cristian, J. B. D. L., Luciana, F. C., Kate, C. B., & Jonas, C. (2009) · African Journal of Biotechnology · doi:10.5897/ajb09.627 · Cited by 46
“Moreover, molasses and corn steep liquor are prominent culture media in fermentative processes due to the high content of sugars and nitrogen, respectively (Beaulieu et al., 1995).”
Background
Metadata
- Authors
- Beaulieu, M., Beaulieu, Y., Melinard, J., Pandian, S., & Goulet, J.
- ORCID
- Beaulieu, M
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1995-01
- Volume
- 61
- Issue
- 1
- Pages
- 165-169
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC1388323
- OpenAlex
- W2146978887
- Cited by
- 94
- References
- 0
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 4 · 2023: 5 · 2022: 2 · 2021: 3 · 2020: 8 · 2019: 6 · 2018: 2 · 2017: 2 · 2016: 6 · 2015: 4 · 2014: 5 · 2013: 3 · 2012: 7
- FWCI
- 0.70
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
- Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Biopolymer Synthesis and Applications
- Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
- Keywords
- polyhydroxybutyrate, Cupriavidus necator, ammonium salts, sugarcane molasses, ammonium sulfate, bacterial growth, PHB production, nitrogen source, glucose fermentation, biomass yield
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