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Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes
Open accessAbstract
We constructed nine sets of oligonucleotide primers on the basis of the results of DNA hybridization of cloned genes from Neurospora crassa and Aspergillus nidulans to the genomes of select filamentous ascomycetes and deuteromycetes (with filamentous ascomycete affiliations). Nine sets of primers were designed to amplify segments of DNA that span one or more introns in conserved genes. PCR DNA amplification with the nine primer sets with genomic DNA from ascomycetes, deuteromycetes, basidiomycetes, and plants revealed that five of the primer sets amplified a product only from DNA of the filamentous ascomycetes and deuteromycetes. The five primer sets were constructed from the N. crassa genes for histone 3, histone 4, beta-tubulin, and the plasma membrane ATPase. With these five primer sets, polymorphisms were observed in both the size of and restriction enzyme sites in the amplified products from the filamentous ascomycetes. The primer sets described here may provide useful tools for phylogenetic studies and genome analyses in filamentous ascomycetes and deuteromycetes (with ascomycete affiliations), as well as for the rapid differentiation of fungal species by PCR.
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Citations
Cited by
The Colletotrichum gloeosporioides species complex
Weir, B. S., Johnston, P. R., & Damm, U. (2012) · Studies in Mycology · doi:10.3114/sim0011 · Cited by 1,646
The Colletotrichum acutatum species complex
Damm, U., Cannon, P. F., Woudenberg, J. H. C., & Crous, P. W. (2012) · Studies in Mycology · doi:10.3114/sim0010 · Cited by 979
Fungal diversity notes 111–252—taxonomic and phylogenetic contributions to fungal taxa
Ariyawansa, H. A., Hyde, K. D., Jayasiri, S. C., Buyck, B., Chethana, K. W. T., Dai, D. Q., Dai, Y. C., Daranagama, D. A., Jayawardena, R. S., Lücking, R., et al. (2015) · Fungal Diversity · doi:10.1007/s13225-015-0346-5 · Cited by 751
The Colletotrichum boninense species complex
Damm, U., Cannon, P. F., Woudenberg, J. H. C., Johnston, P. R., Weir, B. S., Tan, Y. P., Shivas, R. G., & Crous, P. W. (2012) · Studies in Mycology · doi:10.3114/sim0002 · Cited by 485
Slippers, B., Crous, P. W., Denman, S., Coutinho, T. A., Wingfield, B. D., & Wingfield, M. J. (2004) · Mycologia · doi:10.1080/15572536.2005.11833000 · Cited by 429
Metadata
- Authors
- Glass, N. L., & Donaldson, G. C.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1995-04
- Volume
- 61
- Issue
- 4
- Pages
- 1323-1330
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1869907716
- Cited by
- 4,755
- References
- 0
- FWCI
- 0.95
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. https://app.cochat.ai/papers/10.1128/aem.61.4.1323-1330.1995
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- Glass, N. L., & Donaldson, G. C. (1995). Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.61.4.1323-1330.1995
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