The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Green fluorescent protein as a marker for Pseudomonas spp
Open accessAbstract
The development of sensitive methods for observing individual bacterial cells in a population in experimental models and natural environments, such as in biofilms or on plant roots, is of great importance for studying these systems. We report the construction of plasmids which constitutively express a bright mutant of the green fluorescent protein of the jellyfish Aequorea victoria and are stably maintained in Pseudomonas spp. We demonstrate the utility of these plasmids to detect individual cells in two experimental laboratory systems: (i) the examination of a mixed bacterial population of Pseudomonas aeruginosa and Burkholderia cepacia attached to an abiotic surface and (ii) the association of Pseudomonas fluorescens WCS365 with tomato seedling roots. We also show that two plasmids, pSMC2 and pGB5, are particularly useful, because they are stable in the absence of antibiotic selection, they place an undetectable metabolic burden on cells that carry the plasmids, and cells carrying these constructs continue to fluoresce even after 7 days in culture without the addition of fresh nutrients. The construction of improved Escherichia coli-Pseudomonas shuttle vectors which carry multiple drug resistance markers also is described.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Yanisch-Perron, C., Vieira, J., & Messing, J. (1985) · Gene · doi:10.1016/0378-1119(85)90120-9 · Cited by 15,041
Green fluorescent protein as a marker for gene expression
Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W., & Prasher, D. C. (1994) · Science · doi:10.1126/science.8303295 · Cited by 6,802
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.
Bolívar, F. G., Rl, R., Pj, G., Betlach, M. C., Hl, H., Boyer, H. W., Crosa, J. H., & Falkow, S. (1992) · PubMed · Cited by 4,672
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Bolivar, F., Rodriguez, R. L., Greene, P. J., Betlach, M. C., Heyneker, H. L., Boyer, H. W., Crosa, J. H., & Falkow, S. (1977) · PubMed · Cited by 2,750
Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia
Govan, J. R. W., & Deretic, V. P. (1996) · Microbiological Reviews · doi:10.1128/mmbr.60.3.539-574.1996 · Cited by 1,812
Pardee, A. B., Jacob, F., & Monod, J. (1959) · Journal of Molecular Biology · doi:10.1016/s0022-2836(59)80045-0 · Cited by 1,117
MEYER, J. M., & ABDALLAH, M. A. (1978) · Journal of General Microbiology · doi:10.1099/00221287-107-2-319 · Cited by 844
Optical sectioning of microbial biofilms
Lawrence, J. R., Korber, D. R., Hoyle, B. D., Costerton, J. W., & Caldwell, D. E. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.20.6558-6567.1991 · Cited by 828
Promoters in the nodulation region of the Rhizobium leguminosarum Sym plasmid pRL1JI
Spaink, H. P., Okker, R. J. H., Wijffelman, C. A., Pees, E., & Lugtenberg, B. J. J. (1987) · Plant Molecular Biology · doi:10.1007/bf00017984 · Cited by 642
Self-citationSolubilization of rock phosphate by rape
Hoffland, E., Findenegg, G. R., & Nelemans, J. A. (1989) · Plant and Soil · doi:10.1007/bf02280176 · Cited by 493
Cited by
Biofilms: Survival Mechanisms of Clinically Relevant Microorganisms
Donlan, R. M., & Costerton, J. W. (2002) · Clinical Microbiology Reviews · doi:10.1128/cmr.15.2.167-193.2002 · Cited by 6,644
Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development
O'Toole, G. A., & Kolter, R. (1998) · Molecular Microbiology · doi:10.1046/j.1365-2958.1998.01062.x · Cited by 2,697
Self-citationO'Toole, G. A., & Kolter, R. (1998) · Molecular Microbiology · doi:10.1046/j.1365-2958.1998.00797.x · Cited by 2,537
Self-citationHentzer, M., Riedel, K., Rasmussen, T. B., Heydorn, A., Andersen, J. B., Parsek, M. R., Rice, S. A., Eberl, L., Molin, S., Høiby, N., et al. (2002) · Microbiology · doi:10.1099/00221287-148-1-87 · Cited by 1,050
Pseudomonas biofilm formation and antibiotic resistance are linked to phenotypic variation
Drenkard, E., & Ausubel, F. M. (2002) · Nature · doi:10.1038/416740a · Cited by 997
Metadata
- Authors
- Bloemberg, G. V., O'Toole, G. A., Lugtenberg, B. J., & Kolter, R.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1997-11
- Volume
- 63
- Issue
- 11
- Pages
- 4543-4551
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2104043604
- Cited by
- 324
- References
- 30
- FWCI
- 3.94
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Green fluorescent protein as a marker for Pseudomonas spp. https://app.cochat.ai/papers/10.1128/aem.63.11.4543-4551.1997
- The paper
- Bloemberg, G. V., O'Toole, G. A., Lugtenberg, B. J., & Kolter, R. (1997). Green fluorescent protein as a marker for Pseudomonas spp. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.63.11.4543-4551.1997
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
