The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei
Open accessAbstract
Basic features of regulation of expression of the genes encoding the cellulases of the filamentous fungus Trichoderma reesei QM9414, the genes cbh1 and cbh2 encoding cellobiohydrolases and the genes egl1, egl2 and egl5 encoding endoglucanases, were studied at the mRNA level. The cellulase genes were coordinately expressed under all conditions studied, with the steady-state mRNA levels of cbh1 being the highest. Solka floc cellulose and the disaccharide sophorose induced expression to almost the same level. Moderate expression was observed when cellobiose or lactose was used as the carbon source. It was found that glycerol and sorbitol do not promote expression but, unlike glucose, do not inhibit it either, because the addition of 1 to 2 mM sophorose to glycerol or sorbitol cultures provokes high cellulase expression levels. These carbon sources thus provide a useful means to study cellulase regulation without significantly affecting the growth of the fungus. RNA slot blot experiments showed that no expression could be observed on glucose-containing medium and that high glucose levels abolish the inducing effect of sophorose. The results clearly show that distinct and clear-cut mechanisms of induction and glucose repression regulate cellulase expression in an actively growing fungus. However, derepression of cellulase expression occurs without apparent addition of an inducer once glucose has been depleted from the medium. This expression seems not to arise simply from starvation, since the lack of carbon or nitrogen as such is not sufficient to trigger significant expression.
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Cites
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EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme
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Microbial Cellulose Utilization: Fundamentals and Biotechnology
Lynd, L. R., Weimer, P. J., van Zyl, W. H., & Pretorius, I. S. (2002) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.66.3.506-577.2002 · Cited by 4,726
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Metadata
- Authors
- Ilmén, M., Saloheimo, A., Onnela, M. L., & Penttilä, M. E.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1997-04
- Volume
- 63
- Issue
- 4
- Pages
- 1298-1306
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1564184122
- Cited by
- 422
- References
- 28
- FWCI
- 2.48
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Ilmén, M., Saloheimo, A., Onnela, M. L., & Penttilä, M. E. (1997). Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.63.4.1298-1306.1997
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